Transmission of Induced Chromosomal Aberrations through Successive Mitotic Divisions in Human Lymphocytes after In Vitro and In Vivo Radiation
| Autor: | Eric Laplagne, Michelle Ricoul, Elie Maalouf, Georges Deschenes, Mustafa Al Jawhari, William M. Hempel, Akram Kaddour, Michèle El May, Corina Cuceu, Claire Borie, Radhia M'kacher, Eric Jeandidier, Theodore Girinsky, Aude Lenain, Diane Buron, Alain Dieterlen, Annelise Bennaceur-Griscelli, Patrice Carde, Laure Sabatier, Bruno Colicchio, Leonhard Heidingsfelder, Luc Morat |
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| Přispěvatelé: | Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Recherche en Informatique Mathématiques Automatique Signal - IRIMAS - UR 7499 (IRIMAS), Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Modélisation, Intelligence, Processus et Système (MIPS), Modèles de Cellules Souches Malignes et Thérapeutiques, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell division Somatic cell Science Mitosis [SDV.IB.MN]Life Sciences [q-bio]/Bioengineering/Nuclear medicine Biology Giant Cells Article 03 medical and health sciences Dicentric chromosome Chromosome instability Chromosomal Instability Chromosomes Human Humans Lymphocytes Chromosome Aberrations Multidisciplinary Chromosome Reproducibility of Results Cell cycle Telomere Molecular biology 030104 developmental biology [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics Gamma Rays Immunology Medicine |
| Zdroj: | Scientific Reports Scientific Reports, 2017, 7 (1), pp.3291. ⟨10.1038/s41598-017-03198-7⟩ Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
| ISSN: | 2045-2322 |
| Popis: | The mechanisms behind the transmission of chromosomal aberrations (CA) remain unclear, despite a large body of work and major technological advances in chromosome identification. We reevaluated the transmission of CA to second- and third-division cells by telomere and centromere (TC) staining followed by M-FISH. We scored CA in lymphocytes of healthy donors after in vitro irradiation and those of cancer patients treated by radiation therapy more than 12 years before. Our data demonstrate, for the first time, that dicentric chromosomes (DCs) decreased by approximately 50% per division. DCs with two centromeres in close proximity were more efficiently transmitted, representing 70% of persistent DCs in ≥M3 cells. Only 1/3 of acentric chromosomes (ACs), ACs with four telomeres, and interstitial ACs, were paired in M2 cells and associated with specific DCs configurations. In lymphocytes of cancer patients, 82% of detected DCs were characterized by these specific configurations. Our findings demonstrate the high stability of DCs with two centromeres in close proximity during cell division. The frequency of telomere deletion increased during cell cycle progression playing an important role in chromosomal instability. These findings could be exploited in the follow-up of exposed populations. |
| Databáze: | OpenAIRE |
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