Stable Force Balance between Epithelial Cells Arises from F-Actin Turnover
| Autor: | Norbert Perrimon, Michael B. Tworoger, Lizabeth A. Perkins, Elena R. Kingston, Jeanne N. Jodoin, Jonathan S. Coravos, Claudia G. Vasquez, Adam C. Martin, Soline Chanet |
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| Přispěvatelé: | Massachusetts Institute of Technology. Department of Biology, Jodoin, Jeanne, Coravos, Jonathan Stuck, Chanet, Soline, Tworoger, Michael B, Kingston, Elena, Martin, Adam C, Vasquez, Claudia G |
| Rok vydání: | 2015 |
| Předmět: |
Cadherin
Epithelial Cells Septate junctions Adherens Junctions Cell Biology Biology Cadherins Cell junction Actins Epithelium General Biochemistry Genetics and Molecular Biology Cell biology Adherens junction Intercellular Junctions medicine.anatomical_structure medicine Animals Drosophila Cytoskeleton Molecular Biology Intracellular Actin Developmental Biology |
| Zdroj: | PMC |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2015.11.018 |
| Popis: | The propagation of force in epithelial tissues requires that the contractile cytoskeletal machinery be stably connected between cells through E-cadherin-containing adherens junctions. In many epithelial tissues, the cells’ contractile network is positioned at a distance from the junction. However, the mechanism or mechanisms that connect the contractile networks to the adherens junctions, and thus mechanically connect neighboring cells, are poorly understood. Here, we identified the role for F-actin turnover in regulating the contractile cytoskeletal network’s attachment to adherens junctions. Perturbing F-actin turnover via gene depletion or acute drug treatments that slow F-actin turnover destabilized the attachment between the contractile actomyosin network and adherens junctions. Our work identifies a critical role for F-actin turnover in connecting actomyosin to intercellular junctions, defining a dynamic process required for the stability of force balance across intercellular contacts in tissues. National Institute of General Medical Sciences (U.S.) (F32GM113425) National Institute of General Medical Sciences (U.S.) (R01GM084947) National Institute of General Medical Sciences (U.S.) (R01GM105984) |
| Databáze: | OpenAIRE |
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