Increase of reactive oxygen species in different tissues during lipopolysaccharide-induced fever and antipyresis: an electron paramagnetic resonance study

Autor:	Paulo E. N. Souza, Paulo C. Morais, Jardeson S. Jorge, Marina Firmino, Fabiane H. Veiga-Souza, Glória Emília Petto de Souza, Bruna Rafaela Bezerra Gomes, Marcelo Valle de Sousa, Thays M. Rodovalho, Simone Nardin Weis, Maria L. O. Ferreira
Rok vydání:	2018
Předmět:	0301 basic medicine Lipopolysaccharides Male Lipopolysaccharide Fever medicine.medical_treatment Pharmacology medicine.disease_cause Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Brown adipose tissue medicine Animals Antipyretic Rats Wistar Saline chemistry.chemical_classification Reactive oxygen species Electron Spin Resonance Spectroscopy General Medicine Acetaminophen Rats Oxidative Stress 030104 developmental biology medicine.anatomical_structure chemistry Hypothalamus Reactive Oxygen Species 030217 neurology & neurosurgery Oxidative stress medicine.drug
Zdroj:	Free radical research. 52(3)
ISSN:	1029-2470
Popis:	Fever is a regulated increase in body temperature and a component of the acute-phase response, triggered mainly after the invasion of pathogens in the body. Reactive oxygen species (ROS) are generated during the physiological and pathological processes, and can act as both signalling molecules as well as promoters of oxidative stress. Male Wistar rats, pretreated with oral doses of acetaminophen, celecoxib, dipyrone, or ibuprofen 30 min before an intravenous lipopolysaccharide (LPS) or sterile saline injection, showed a reduced febrile response in all animals tested. The formation of ROS in the fresh blood, liver, brown adipose tissue (BAT), and hypothalamus of febrile and antipyretic-treated animals was assessed by electron paramagnetic resonance using the spin probe 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine (CMH). While the CM• concentrations remained unaltered in the blood samples examined 5 h after the induction of fever, we found increased CM• levels in the liver (in µM, saline: 290 ± 42; LPS: 512 ± 34), BAT (in µM, saline: 509 ± 79, LPS: 855 ± 79), and hypothalamus (in µM, saline: 292 ± 35; LPS: 467 ± 8) at the same time point. Importantly, none of the antipyretics were seen to alter the CM• accumulation profile. Data from this study suggest that there is an increased formation of ROS in the different tissues during fever, which may cause oxidative stress, and that the antipyretics tested do not interfere with ROS production.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f943b99d53df6ea0ed5fbcbb05cc2441 https://pubmed.ncbi.nlm.nih.gov/29308684 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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