Metformin mitigates PLCε gene expression and modulates the Notch1/Hes and androgen receptor signaling pathways in castration-resistant prostate cancer xenograft models
| Autor: | Jianming Pu, Jianguo Tian, Qi Li, Ke Xu, Zhicheng Lu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
PLCε
Cancer Research patient-derived xenotransplantation Oncogene Chemistry Androgen receptor signaling pathway Notch1/Hes signaling pathway Cancer Articles medicine.disease urologic and male genital diseases Metformin Androgen receptor Prostate cancer Oncology Neurogenic Locus Notch Homolog Protein 1 medicine Cancer research CRPC Signal transduction metformin androgen receptor signaling pathway medicine.drug |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 |
| Popis: | The present study aimed to establish a mouse model of patient-derived castration-resistant prostate cancer (CRPC) xenograft tumors, and to evaluate the effects of various doses of metformin on phospholipase Ce (PLCe) expression and the neurogenic locus notch homolog protein 1 (Notch1)/hairy and enhancer of split 1 and androgen receptor (AR) signaling pathways via western blotting and reverse transcription-quantitative PCR. Additionally, phorbol 12-myristate 13-acetate was used to activate PLC, and Jagged1 was used as a Notch activator to verify whether metformin could suppress CRPC development via the PLCe/Notch1/AR pathways. The results confirmed that metformin may serve critical roles in CRPC by significantly inhibiting the occurrence, growth and proliferation of CRPC tumors by decreasing PLCe/Notch1 expression and AR nucleation. |
| Databáze: | OpenAIRE |
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