| Autor: |
Flávia Thamiris Figueiredo Pacheco, Humberto Fonseca de Freitas, Renata Kelly Novais Rodrigues Silva, Silvia Souza de Carvalho, Adson Santos Martins, Joelma Figueiredo Menezes, Tereza Cristina Medrado Ribeiro, Ângela Peixoto de Mattos, Hugo da Costa-Ribeiro Júnior, Joice Neves Reis Pedreira, Neci Matos Soares, Márcia Cristina Aquino Teixeira |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Revista da Sociedade Brasileira de Medicina Tropical, Volume: 55, Article number: e0041-2022, Published: 25 JUL 2022 |
| ISSN: |
1678-9849 |
| Popis: |
Background: Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species. Methods: Children’s feces were analyzed by modified Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain reaction-restriction fragment length polymorphism for species identification. Results: Cryptosporidium frequency was 2.6%. The sensitivity and specificity of ELISA were 85.7% and 99.7%, respectively, with excellent concordance with mZN (kappa=0.854). Parasite species were characterized as Cryptosporidium hominis (78.3%), Cryptosporidium felis (17.4%), and Cryptosporidium parvum (4.3%). Conclusions: Coproantigen ELISA is as efficient as mZN for Cryptosporidium diagnosis. Cryptosporidium genotyping suggests anthroponotic and zoonotic transmission to children. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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