Effect of Rice Cell-Derived Human Granulocyte-Macrophage Colony-Stimulating Factor on 5-Fluorouracil-Induced Mucositis in Hamsters
| Autor: | Hae Chan Ha, Hye-Mi Kim, Dae Kyong Kim, Jong Min Choi, Kyong Hoon Ahn, Chang Koo Yun, Jong Hoon Won, Seok Kyun Kim, Kyuboem Han, Jung Eun Ji |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Antimetabolites Antineoplastic Interleukin-1beta Pharmaceutical Science Hamster Pharmacology Transforming Growth Factor beta Cheek pouch Cricetinae Proliferating Cell Nuclear Antigen Mucositis Animals Medicine Macrophage Oral mucosa Peroxidase Stomatitis Wound Healing Mesocricetus biology business.industry Mouth Mucosa Granulocyte-Macrophage Colony-Stimulating Factor Oryza General Medicine biology.organism_classification medicine.disease Recombinant Proteins medicine.anatomical_structure Granulocyte macrophage colony-stimulating factor Immunology Fluorouracil business Wound healing medicine.drug |
| Zdroj: | Biological and Pharmaceutical Bulletin. 36:425-431 |
| ISSN: | 1347-5215 0918-6158 |
| Popis: | Granulocyte-macrophage colony-stimulating factor (GM-CSF) is an important regulator of the maturation and function of cells in the granulocyte and macrophage lineages, and also plays a significant role in wound healing. In a previous study, we expressed human GM-CSF in rice cells (rice cell-derived human GM-CSF; rhGM-CSF). The purpose of the present study was to evaluate its effect on wound healing in oral mucositis. Oral mucositis was induced in Syrian hamster cheek pouches by 5-fluorouracil treatment and mechanical scratching. Ulcerated areas were treated from days 3 to 14 with an application of 200 µL saline, or of the same volume of a solution containing 0.04, 0.2, or 1 µg/mL rhGM-CSF. Treatment of hamsters with rhGM-CSF reduced the ulcerated areas of the oral mucosa, compared with the control. Early in the healing process, the mucositis tissue layer of the rhGM-CSF-treated group showed significantly decreased myeloperoxidase activity and increased numbers of proliferating cell nuclear antigen (PCNA)-positive cells. Treatment with rhGM-CSF also affected expression of inflammatory cytokines in the ulcerative mucosal tissue. These results demonstrate the efficacy of plant-produced rhGM-CSF in wound healing and have significant implications for the development of rhGM-CSF as a therapeutic agent for ulcerative oral mucositis. |
| Databáze: | OpenAIRE |
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