Altered calcium handling and increased contraction force in human embryonic stem cell derived cardiomyocytes following short term dexamethasone exposure
| Autor: | Marcelo C. Ribeiro, Simona Casini, Berend J. van Meer, Christine L. Mummery, Robert Passier, Dorien Ward-van Oostwaard, Georgios Kosmidis, Milena Bellin, Leon G.J. Tertoolen |
|---|---|
| Přispěvatelé: | Cardiology, Applied Stem Cell Technologies |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Calcium handling Cardiomyocytes Electrophysiology Glucocorticoids Human embryonic stem cells Calcium Cell Line Dexamethasone Embryonic Stem Cells Homeodomain Proteins Humans Myocytes Cardiac Sarcoplasmic Reticulum Calcium-Transporting ATPases Signal Transduction Biophysics chemistry.chemical_element Biology Biochemistry Contractility Internal medicine medicine Induced pluripotent stem cell Molecular Biology health care economics and organizations Myocytes Cell Biology Embryonic stem cell Endocrinology chemistry Cell culture Signal transduction Cardiac Glucocorticoid medicine.drug |
| Zdroj: | Biochemical and biophysical research communications, 467(4), 998-1005. Academic Press Inc. Biochemical and Biophysical Research Communications, 467(4), 998-1005 Biochemical and biophysical research communications, 467(4), 998-1005. Academic Press |
| ISSN: | 0006-291X |
| Popis: | One limitation in using human pluripotent stem cell derived cardiomyocytes (hPSC-CMs) for disease modeling and cardiac safety pharmacology is their immature functional phenotype compared with adult cardiomyocytes. Here, we report that treatment of human embryonic stem cell derived cardiomyocytes (hESC-CMs) with dexamethasone, a synthetic glucocorticoid, activated glucocorticoid signaling which in turn improved their calcium handling properties and contractility. L-type calcium current and action potential properties were not affected by dexamethasone but significantly faster calcium decay, increased forces of contraction and sarcomeric lengths, were observed in hESC-CMs after dexamethasone exposure. Activating the glucocorticoid pathway can thus contribute to mediating hPSC-CMs maturation. |
| Databáze: | OpenAIRE |
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