On the catalytic mechanism of bacteriophage endolysins: Opportunities for engineering
| Autor: | Gayan Abeysekera, Renwick C. J. Dobson, Andrew C. Muscroft-Taylor, Michael J. Love, Craig Billington |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
030306 microbiology Mechanism (biology) Hydrolases Biophysics Lysin Protein engineering Computational biology Biology biology.organism_classification Protein Engineering Biochemistry Catalysis Analytical Chemistry Anti-Bacterial Agents Bacteriophage 03 medical and health sciences Bacteriophages Molecular Biology Function (biology) 030304 developmental biology |
| Zdroj: | Biochimica et biophysica acta. Proteins and proteomics. 1868(1) |
| ISSN: | 1878-1454 |
| Popis: | Bacteriophage endolysins have the potential to be a long-term antibacterial replacement for antibiotics. The exogenous application of endolysins on some bacteria results in rapid cell lysis. The prospects for endolysins are furthered by the ability to engineer them; novel endolysins can be developed with optimised stability, specificity, and lytic function. But the success of endolysin engineering and application requires a comprehensive understanding of the relationship between the enzymes biochemical, biophysical and bacteriolytic properties. Here, we examine their catalytic mechanisms, opportunities for developing novel endolysins, and highlight areas where a better understanding would support their long-term success as antibacterial agents. |
| Databáze: | OpenAIRE |
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