Molecular cloning and variation of ω-gliadin genes from a somatic hybrid introgression line II-12 and parents (Triticum aestivum cv. Jinan 177 and Agropyron elongatum)
| Autor: | Guangmin Xia, Fanguo Chen, Liang Yang, Haoxiang Min, Feng Zhao |
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| Rok vydání: | 2011 |
| Předmět: |
Genetics
Signal peptide Molecular Sequence Data nutritional and metabolic diseases food and beverages Genetic Variation Sequence Analysis DNA Biology Molecular cloning Hybrid Cells Gliadin Polyploidy Somatic fusion biology.protein Gene family Coding region Amino Acid Sequence Cloning Molecular Agropyron Gene Peptide sequence Phylogeny Triticum |
| Zdroj: | Journal of genetics. 90(1) |
| ISSN: | 0973-7731 |
| Popis: | Gluten proteins, including gliadins and glutenins, together are responsible in large part for the end-use quality of the flour, have been extensively studied (Shewry and Tatham 1997; Shewry et al. 2003). The gliadins are monomeric polypeptides, classified into α-, β-, γandωtypes (Shewry et al. 2003). The ω-gliadin genes are encoded by the Gli-1 loci on the short arm of chromosome 1 which is tightly linked to Glu-3 loci, at which the LMW-GS are encoded (Tatham and Shewry 1995). There are also a few ω-gliadins encoded by ‘selfish genes’ at the Gli-A3, Gli-A5, Gli-B5 and Gli-A6 loci (Shewry et al. 2003). The number of ω-gliadins present in the hexaploid wheat endosperm ranges from 15 to 18 (Sabelli and Shewry 1991), and have been classified, according to the first three peptide residues present in the mature protein into ARQ/E-, KEL-, SRLor TRQ-types. ARQ/ E-type shares a 19-residue putative signal peptide, followed by a 10–11-residue N-terminal domain, a large repetitive region (encompassing 90–96% of the protein), and a 10– 11-residue C-terminal domain (Hsia and Anderson 2001). ω-Gliadins are deficient in both cysteine and methionine, and are therefore ‘sulphur poor’ polypeptides (Tatham and Shewry 1995). However, rather few ω-gliadin genes are represented (Hsia and Anderson 2001; Masoudi-Nejad et al. 2002; Matsuo et al. 2005; Hassania et al. 2008), possibly because their coding sequence includes a large repetitive domain, which hampers cloning. Further analysis ofω-gliadins at the DNA level would provide more information to define the evolution and function of this gene family (Hassania et al. 2008). |
| Databáze: | OpenAIRE |
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