Downregulation of glutamatergic and GABAergic proteins in valproric acid associated social impairment during adolescence in mice
| Autor: | Angus Yiu-Ting Choi, Wing Nang Leung, Davor Kin-Fan Chau, Wen Yang, Chun Wai Chan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Time Factors GABA Agents GABRA5 Glutamate decarboxylase Down-Regulation Inhibitory postsynaptic potential gamma-Aminobutyric acid Electron Transport Complex IV Mice 03 medical and health sciences Behavioral Neuroscience Glutamatergic 0302 clinical medicine Receptors GABA Pregnancy Internal medicine medicine Animals Social Communication Disorder Cerebral Cortex Valproic Acid biology Brain-Derived Neurotrophic Factor 030104 developmental biology Endocrinology Animals Newborn Receptors Glutamate Prenatal Exposure Delayed Effects Linear Models Excitatory postsynaptic potential biology.protein NMDA receptor Female Rabbits Psychology Neuroscience Locomotion 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Behavioural Brain Research. 316:255-260 |
| ISSN: | 0166-4328 |
| DOI: | 10.1016/j.bbr.2016.09.003 |
| Popis: | The etiology of Autism Spectrum Disorder (ASD) remains controversial. Deficits in social communication are one of the key criteria for ASD diagnosis. Valproic acid (VPA), which is an anti-epileptic and anti-depressive drug, is one of the teratogens to cause ASD onset. Moreover, synaptic dysfunction is suggested as one of the major causative factor in VPA-induced ASD in vitro and in vivo studies. Herein, this study aimed to determine the excitatory/inhibitory synaptic mRNA and protein expression in VPA-induced autistic mice. Pregnant BALB/c mice were injected peritoneally with a single dose of 600mg/kg VPA on embryonic day (E) 12.5. Social impairment was verified by three chamber sociability tests on postnatal days (PND) 28, 35, 42 and 49. Cortical synaptic mRNA and protein expressions were examined on PND 50. The excitatory synaptic proteins NR2A, NR2B, NR2C were significantly down-regulated by 80.0% (p |
| Databáze: | OpenAIRE |
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