Microencapsulation of living cells in semi-permeable membranes with covalently cross-linked layers
| Autor: | Nathalie Henley, François A. Leblond, Martin Ménard, Robert Robitaille, Guillaume Jourdan, Jean-Pierre Hallé, Julie Dusseault, Josée Tessier |
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| Rok vydání: | 2005 |
| Předmět: |
Pancreas
Artificial Materials science Compressive Strength Alginates Cell Survival Cell Culture Techniques Islets of Langerhans Transplantation Biophysics Bioengineering Permeability Buffer (optical fiber) Biomaterials Islets of Langerhans chemistry.chemical_compound Coated Materials Biocompatible Phenyl azide Materials Testing Polymer chemistry Animals Polylysine Viability assay Semipermeable membrane Rats Wistar Cells Cultured Tissue Engineering Membranes Artificial Rats Molecular Weight Transplantation Cross-Linking Reagents Membrane Chemical engineering chemistry Mechanics of Materials Covalent bond Permeability (electromagnetism) Ceramics and Composites |
| Zdroj: | Biomaterials. 26:1515-1522 |
| ISSN: | 0142-9612 |
| Popis: | Microencapsulation in semi-permeable membranes protects transplanted cells against immune destruction. Microcapsule strength is critical. We describe a method to microencapsulate living cells in alginate-poly-L-lysine (PLL)-alginate membranes with covalent links between adjacent layers of microcapsule membranes, while preserving the desired membrane molecular weight cut-off (MWCO) and microencapsulated cell viability. A heterobifunctional photoactivatable cross-linker, N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) was used. The N-hydroxysuccinimide ester group of ANB-NOS was covalently linked to PLL. Islets of Langerhans were immobilized in alginate beads, incubated in PLL-ANB-NOS and again in alginate. Upon illumination with UVA, covalent links were created between the phenyl azide residue of ANB-NOS and alginate from both the core bead and the outer coating. Covalently linked microcapsules remained intact after 3 years in a strong alkaline buffer (pH 12), whereas standard microcapsules disappeared within 45 s in the same solution. A standardized mechanical stress broke 22-fold more standard than covalently linked microcapsules. The MWCO and microencapsulated cell viability were similar with standard and covalently linked microcapsules. These microcapsules, extremely resistant to chemical and mechanical stresses, will be useful in numerous applications. |
| Databáze: | OpenAIRE |
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