An intron within the 16S ribosomal RNA gene of the archaeon Pyrobaculum aerophilum
| Autor: | C R Woese, Siegfried Burggraf, Karl O. Stetter, N Larsen |
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| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Biology DNA Ribosomal Polymerase Chain Reaction Homology (biology) Open Reading Frames RNA Ribosomal 16S Amino Acid Sequence Gene Phylogeny Genetics Multidisciplinary Base Sequence Intron RNA Ribosomal RNA Archaea Biological Evolution Introns Open reading frame Oligodeoxyribonucleotides Genes Bacterial Protein Biosynthesis Transfer RNA RNA splicing Nucleic Acid Conformation Research Article |
| Popis: | The 16S rRNA genes of Pyrobaculum aerophilum and Pyrobaculum islandicum were amplified by the polymerase chain reaction, and the resulting products were sequenced directly. The two organisms are closely related by this measure (over 98% similar). However, they differ in that the (lone) 16S rRNA gene of Pyrobaculum aerophilum contains a 713-bp intron not seen in the corresponding gene of Pyrobaculum islandicum. To our knowledge, this is the only intron so far reported in the small subunit rRNA gene of a prokaryote. Upon excision the intron is circularized. A secondary structure model of the intron-containing rRNA suggests a splicing mechanism of the same type as that invoked for the tRNA introns of the Archaea and Eucarya and 23S rRNAs of the Archaea. The intron contains an open reading frame whose protein translation shows no certain homology with any known protein sequence. |
| Databáze: | OpenAIRE |
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