Structural optimization of cyclic peptides that efficiently detect denatured collagen

Autor: Kento Ishii, Kazunori K. Fujii, Takaki Koide, Koh K. Takita
Rok vydání: 2019
Předmět:
Zdroj: Organicbiomolecular chemistry. 17(31)
ISSN: 1477-0539
Popis: To develop a facile method for detecting denatured collagen, we investigated the structure-activity relationship of cyclic collagen-mimetic peptides (cCMPs). Reported cCMP prototypes tend to self-assemble and they must be disassembled just before use. Introducing charge repulsion and a deformation in the peptide backbone structure enabled cCMPs to detect denatured collagen without a pre-treatment for disassembly. Using the optimized cCMP, types I-V collagen were detected by western blotting and denatured collagen fibrils were visualized in a cell culture system.
Databáze: OpenAIRE