Structural optimization of cyclic peptides that efficiently detect denatured collagen
| Autor: | Kento Ishii, Kazunori K. Fujii, Takaki Koide, Koh K. Takita |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
CCMP
02 engineering and technology Biochemistry Peptides Cyclic Collagen fibril 03 medical and health sciences Mice Structure-Activity Relationship Structure–activity relationship Animals Physical and Theoretical Chemistry Cells Cultured 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chemistry Organic Chemistry Fibroblasts 021001 nanoscience & nanotechnology Cyclic peptide Blot Peptide backbone Cell culture Biophysics Collagen 0210 nano-technology |
| Zdroj: | Organicbiomolecular chemistry. 17(31) |
| ISSN: | 1477-0539 |
| Popis: | To develop a facile method for detecting denatured collagen, we investigated the structure-activity relationship of cyclic collagen-mimetic peptides (cCMPs). Reported cCMP prototypes tend to self-assemble and they must be disassembled just before use. Introducing charge repulsion and a deformation in the peptide backbone structure enabled cCMPs to detect denatured collagen without a pre-treatment for disassembly. Using the optimized cCMP, types I-V collagen were detected by western blotting and denatured collagen fibrils were visualized in a cell culture system. |
| Databáze: | OpenAIRE |
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