Shedding Light on the Antimicrobial Peptide Arsenal of Terrestrial Isopods: Focus on Armadillidins, a New Crustacean AMP Family
| Autor: | Christine Braquart-Varnier, Thomas Becking, Richard Cordaux, Carine Delaunay, Julien Verdon, Jean-Marc Berjeaud |
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| Přispěvatelé: | Ecologie et biologie des interactions (EBI), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
animal structures lcsh:QH426-470 [SDV]Life Sciences [q-bio] Antimicrobial peptides glycine-rich peptides MESH: Sequence Alignment Zoology MESH: Amino Acid Sequence Article 03 medical and health sciences antimicrobial peptides transcriptomics Malacostraca Genetics Animals MESH: Animals MESH: Cloning Molecular 14. Life underwater Amino Acid Sequence Cloning Molecular MESH: Phylogeny innate immunity Genetics (clinical) Phylogeny Armadillidium vulgare Innate immune system 030102 biochemistry & molecular biology biology fungi MESH: Antimicrobial Cationic Peptides malacostraca terrestrial isopods MESH: Transcription Factors crustacea Crayfish biology.organism_classification Antimicrobial Crustacean Shrimp lcsh:Genetics 030104 developmental biology MESH: Isopoda Sequence Alignment Antimicrobial Cationic Peptides Isopoda Transcription Factors |
| Zdroj: | Genes Genes, MDPI, 2020, 11 (1), ⟨10.3390/genes11010093⟩ Volume 11 Issue 1 Genes, Vol 11, Iss 1, p 93 (2020) |
| ISSN: | 2073-4425 |
| DOI: | 10.3390/genes11010093⟩ |
| Popis: | International audience; In crustaceans, antimicrobial peptides (AMPs) are clustered into four major groups according to their amino acid composition and structure: (1) single-domain peptides containing cysteine residues such as anti-lipopolysaccharide-factor (ALF), (2) multi-domain or chimeric AMPs such as crustins, (3) non-conventional AMPs, and (4) linear single-domain AMPs. The majority of AMPs has been described in commercially exploited crustaceans, particularly decapods living in aquatic environments (crab, shrimp, lobster, and crayfish). Here, we aimed at establishing the AMPs repertoire of terrestrial isopods (Oniscidea), an original suborder of crustaceans adapted to life outside of the aquatic environment. Using transcriptomic data from 21 species, we identified 110 ALF and 73 crustin sequences. We also characterized the full-length sequence of armadillidins from 17 species, similar to the AMP previously described in the terrestrial isopod Armadillidium vulgare. Furthermore, we tested the antimicrobial activity of three armadillidin peptides characterized from three distantly related species. This analysis revealed similar activity spectra against pathogens, despite extensive structural variation among the tested peptides. In addition to conventional crustacean AMPs, our work highlights armadillidins as a new and independent family of AMPs specific to the Oniscidea, thus opening new perspectives concerning the study of the immune system of terrestrial isopods. |
| Databáze: | OpenAIRE |
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