Transcriptome sequencing analysis of primary fibroblasts: a new insight into postoperative abdominal adhesion
| Autor: | Wenqin Liu, Lianbing Hou, Chuqi Hou, Yilei Li, Qin Yang, Canmao Wang, Fuling Wu |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Interleukin-1beta Tissue Adhesions Masson's trichrome stain Transcriptome Pathogenesis Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Immune system Postoperative Complications Downregulation and upregulation Fibrosis Medicine Animals Humans Molecular Targeted Therapy Insulin-Like Growth Factor I Gene CD11b Antigen business.industry Sequence Analysis RNA Tumor Necrosis Factor-alpha General Medicine Fibroblasts medicine.disease ErbB Receptors Disease Models Animal Receptors Granulocyte-Macrophage Colony-Stimulating Factor 030220 oncology & carcinogenesis Cancer research 030211 gastroenterology & hepatology Surgery Tumor necrosis factor alpha Peritoneum business |
| Zdroj: | Surgery today. 52(1) |
| ISSN: | 1436-2813 |
| Popis: | The specific genes or pathways in fibroblasts responsible for the pathogenesis of postoperative abdominal adhesion (PAA) remain to be elucidated. We aim to provide a new insight into disease mechanisms at the transcriptome level. Male Sprague–Dawley rats were used to establish a PAA model. Primary fibroblasts were separated from normal peritoneal tissue (NF) and postoperative adhesion tissue (PF). RNA sequencing was used to analyze the transcriptome in NF and PF. One thousand two hundred thirty-five upregulated and 625 downregulated DEGs were identified through RNA-Seq. A pathway enrichment analysis identified distinct enriched biological processes, among which the most prominent was related to immune and inflammatory response and fibrosis. HE staining and Masson’s trichrome staining histologically validated the RNA-Seq results. Six hub genes, ITGAM, IL-1β, TNF, IGF1, CSF1R and EGFR were further verified by RT-PCR. Our study revealed the roles of the immune and inflammatory responses and fibrosis in the process of PAA. We also found six hub genes that may be potential therapeutic targets for PPA. |
| Databáze: | OpenAIRE |
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