A Genetic Linkage Map of the Baboon (Papio hamadryas) Genome Based on Human Microsatellite Polymorphisms

Autor: Jeffrey Rogers, Lawrence A. Rodriguez, Karen E. Chambers, Susan H. Slifer, Phillip A. Morin, Karen Rice, Michael C. Mahaney, Pauline Parry, Andrey A. Perelygin, Shalini Nair, Paula Palladino-Negro, Timothy K. Newman, Michael Slifer, Deborah E. Newman, Shelly M. Witte, Steven Wedel, Geoff Joslyn
Rok vydání: 2000
Předmět:
Zdroj: Genomics. 67:237-247
ISSN: 0888-7543
DOI: 10.1006/geno.2000.6245
Popis: A first-generation genetic linkage map of the baboon (Papio hamadryas) genome was developed for use in biomedical and evolutionary genetics. Pedigreed baboons (n = 694) were selected from the breeding colony maintained by the Southwest Foundation for Biomedical Research. To facilitate comparison with the human genome, the baboon linkage map consists primarily of human microsatellite loci amplified using published human PCR primers. Genotypes for 325 human microsatellites and 6 novel baboon microsatellites were used in linkage analyses performed with the MultiMap expert system. The resulting sex-averaged meiotic recombination map covers all 20 baboon autosomes, with average spacing among loci of 7.2 cM. Direct comparison among homologous (orthologous) loci reveals that, for 7 human autosomes, locus order is conserved between humans and baboons. For the other 15 autosomes, one or more rearrangements distinguish the two genomes. The total centimorgan distances among homologous markers are 28.0% longer in the human genome than in the baboon, suggesting that rates of recombination may be higher in humans. This baboon linkage map is the first reported for any nonhuman primate species and creates opportunities for mapping quantitative trait loci in baboons, as well as for comparative evolutionary analyses of genome structure.
Databáze: OpenAIRE
Externí odkaz: