Proton NMR Spectroscopy of Glucose Consumption by Cultured Lens Epithelial Cells
| Autor: | Dominic Man-Kit Lam, Joseph J. Ford, Eric Aguiar, Peter J. Kelleher, Hong-Ming Cheng |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Pharmacology
Magnetic Resonance Spectroscopy Chemistry Pentose phosphate pathway Molecular biology Epithelium Pentose Phosphate Pathway Proton nmr spectroscopy Ophthalmology Proton resonance Glucose medicine.anatomical_structure Biochemistry Lens (anatomy) Lens Crystalline Lactates medicine Animals Pharmacology (medical) Lactic Acid Rabbits Cells Cultured |
| Zdroj: | Journal of Ocular Pharmacology and Therapeutics. 2:319-324 |
| ISSN: | 1557-7732 1080-7683 |
| DOI: | 10.1089/jop.1986.2.319 |
| Popis: | Proton NMR spectroscopy was performed on media collected from cultured lens epithelial cells of the rabbit eye incubated with Krebs-Ringer's solution containing 5.5mM C-glucose (labeled at the C-1 position). Comparing proton resonance intensities of the lactate-methyl group of the C-3 carbon (both 12C and 13C) enabled us to quantify the hexose monophosphate shunt (HMPS) activity. Our results showed that the epithelial cells remained stable in both lactate production and HMPS activity for at least 8 hours. In addition, although tBHP (1mM) resulted in an increase of glucose flow through the HMPS, the rate of lactate production was not affected. In contrast, KCN (2mM) caused a 72% increase of lactate production and a slight (6%) decrease of glucose consumption through the HMPS. |
| Databáze: | OpenAIRE |
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