Use of TaqMan Array Cards to Screen Outbreak Specimens for Causes of Febrile Illness in Tanzania
| Autor: | Ireen Kiwelu, James J. Gibson, Jean Gratz, Maria E. Kelly, Fausta Mosha, Eric R. Houpt, Rachel B. Eidex, Buliga Mujaga, Blandina T. Mmbaga, Ahmed Abade, Jie Liu, Athanasia Maro |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Diagnostic Screening Programs Male Fever 030106 microbiology Real-Time Polymerase Chain Reaction Communicable Diseases Tanzania Typhoid fever Disease Outbreaks Diagnosis Differential 03 medical and health sciences 0302 clinical medicine Risk Factors Virology medicine TaqMan Prevalence Humans 030212 general & internal medicine Child biology business.industry Febrile illness Outbreak Articles medicine.disease biology.organism_classification Infectious Diseases Case-Control Studies Parasitology Christian ministry Female Medical emergency business National laboratory |
| Zdroj: | The American journal of tropical medicine and hygiene. 98(6) |
| ISSN: | 1476-1645 |
| Popis: | We describe the deployment of a custom-designed molecular diagnostic TaqMan Array Card (TAC) to screen for 31 bacterial, protozoal, and viral etiologies in blood from outbreaks of acute febrile illness in Tanzania during 2015-2017. On outbreaks notified to the Tanzanian Ministry of Health, epidemiologists were dispatched and specimens were collected, transported to a central national laboratory, and tested by TAC within 2 days. This algorithm streamlined investigation, diagnosed a typhoid outbreak, and excluded dozens of other etiologies. This method is usable in-country and may be incorporated into algorithms for diagnosing outbreaks. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|