Regulation of Endogenous Human Gene Expression by Ligand-Inducible TALE Transcription Factors
| Autor: | Shannon J. Sirk, Carlos F. Barbas, Brian M. Lamb, Thomas Gaj, Andrew C. Mercer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Recombinant Fusion Proteins
Response element Biomedical Engineering Gene regulatory network E-box Biology Ligands Protein Engineering Biochemistry Genetics and Molecular Biology (miscellaneous) steroid hormone receptor 03 medical and health sciences 0302 clinical medicine TALE Humans Gene Regulatory Networks Enhancer Transcription factor transcription factor 030304 developmental biology Cis-regulatory module Genetics Regulation of gene expression 0303 health sciences Base Sequence Promoter General Medicine DNA Cell biology HEK293 Cells Gene Expression Regulation Receptors Estrogen Trans-Activators Synthetic Biology inducible gene expression gene regulation Receptors Progesterone 030217 neurology & neurosurgery Research Article HeLa Cells |
| Zdroj: | ACS Synthetic Biology |
| ISSN: | 2161-5063 |
| Popis: | The construction of increasingly sophisticated synthetic biological circuits is dependent on the development of extensible tools capable of providing specific control of gene expression in eukaryotic cells. Here, we describe a new class of synthetic transcription factors that activate gene expression in response to extracellular chemical stimuli. These inducible activators consist of customizable transcription activator-like effector (TALE) proteins combined with steroid hormone receptor ligand-binding domains. We demonstrate that these ligand-responsive TALE transcription factors allow for tunable and conditional control of gene activation and can be used to regulate the expression of endogenous genes in human cells. Since TALEs can be designed to recognize any contiguous DNA sequence, the conditional gene regulatory system described herein will enable the design of advanced synthetic gene networks. |
| Databáze: | OpenAIRE |
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