CD146 expression regulates osteochondrogenic differentiation of human adipose-derived stem cells
| Autor: | Augusto Orlandi, Maria Giovanna Scioli, Manuel Scimeca, Gabriele Storti, Jeffrey M. Gimble, Massimo Sanchez, Valerio Cervelli, Alessandra Bielli |
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| Rok vydání: | 2021 |
| Předmět: |
endocrine system
Physiology animal diseases Clinical Biochemistry Population Adipose tissue tissue regeneration CD146 Antigen Biology Tissue engineering Adipocytes Humans education cell sorting Cells Cultured Matrigel education.field_of_study Stem Cells Settore MED/19 hemic and immune systems Cell Differentiation Cell Biology differentiation Chondrogenesis eye diseases Cell biology Adipose Tissue Adipogenesis CD146 human adipose-derived stem cells Stem cell tissues |
| Zdroj: | Journal of cellular physiologyREFERENCES. 237(1) |
| ISSN: | 1097-4652 |
| Popis: | Tissue engineering aims to develop innovative approaches to repair tissue defects. The use of adipose-derived stem cells (ASCs) in tissue regeneration was extensively investigated for osteochondrogenesis. Among the ASC population, ASCs expressing the CD146 were demonstrated to be multipotent and considered as perivascular stem cells, although the functional role of CD146 expression in these cells remains unclear. Herein, we investigated the influence of CD146 expression on osteochondrogenic differentiation of ASCs. Our results showed that, in two-dimensional culture systems, sorted CD146+ ASCs proliferated less and displayed higher adipogenic and chondrogenic potential than CD146- ASCs. The latter demonstrated a higher osteogenic capacity. Besides this, CD146+ ASCs in three-dimensional Matrigel/endothelial growth medium (EGM) cultures showed the highest angiogenic capability. When cultured in three-dimensional collagen scaffolds, CD146+ ASCs showed a spontaneous chondrogenic differentiation, further enhanced by the EGM medium's addition. Finally, CD146- ASCs seeded on hexafluoroisopropanol silk scaffolds displayed a greater spontaneous osteogenetic capacity. Altogether, these findings demonstrated a functional and relevant influence of CD146 expression in ASC properties and osteochondrogenic commitment. Exploiting the combination of specific differentiation properties of ASC subpopulations and appropriate culture systems could represent a promising strategy to improve the efficacy of new regenerative therapies. |
| Databáze: | OpenAIRE |
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