Determination of the Minimum Inhibitory Concentrations of Alexidine and Chlorhexidine Against Enterococcus faecalis and Candida albicans: An In Vitro Study
| Autor: | Atakan Kalender, Mehmet Kiyan, Ece Irem Oguz, Meltem Dartar Öztan, Umut Aksoy, Fatma Kermeoglu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endodontic irrigants e. faecalis Root canal c. albicans Enterococcus faecalis Microbiology Agar plate 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Alexidine Candida albicans biology business.industry chlorhexidine Chlorhexidine General Engineering 030206 dentistry alexidine biology.organism_classification Corpus albicans McFarland standards 030104 developmental biology medicine.anatomical_structure chemistry Other business medicine.drug |
| Zdroj: | Cureus |
| ISSN: | 2168-8184 |
| DOI: | 10.7759/cureus.2221 |
| Popis: | Introduction The root canal system must be mechanically instrumented and chemically cleaned using various antimicrobial irrigants in a sequential manner or in combination for the elimination of necrotic pulp tissue and reducing the number of root canal bacteria. For this reason, new methods and materials are continuously being developed to achieve the objectives of endodontic treatment. Materials and methods E. faecalis (ATCC 29212) and C. albicans (ATCC 90028) standard strains were used for this study. Colonies of E. faecalis and C. albicans were harvested from the agar plates and suspended in 4 mL of phosphate buffered saline (PBS). Microorganisms were diluted to obtain a suspension of approximately 108 colony-forming units/mL (CFU/mL) in sterile PBS using McFarland standard tubes no. 0.5. Results After a two-minute contact time, all alexidine (ALX) concentrations used in this study eradicated all E. faecalis strains, while chlorhexidine (CHX) didn't kill 100% of E. faecalis at 0.25% and lower concentrations even after a five-minute contact time. ALX also eradicated C. albicans at all concentrations even after a one-minute contact time. CHX showed antifungal activity against C. albicans at all concentrations higher than 0.031% after a one-minute contact time. Conclusion A 0.0156% concentration of ALX can be a good alternative to CHX as an irrigation solution in endodontic treatment when used for one minute against E. faecalis and C. albicans. |
| Databáze: | OpenAIRE |
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