Expression and distribution of fugu TIMP-2s (fgTIMP-2a and fgTIMP-2b) mRNAs in tissues and embryos
| Autor: | Shoshi Mizuta, Reiji Yoshinaka, Tohru Suzuki, Yoshihiro Yokoyama, Hiroshi Tsukamoto |
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| Rok vydání: | 2007 |
| Předmět: |
Tissue Inhibitor of Metalloproteinase-2
Messenger RNA Embryo Nonmammalian Base Sequence Takifugu rubripes Physiology Fugu Embryogenesis Embryonic Development Embryo In situ hybridization Biology biology.organism_classification Polymerase Chain Reaction Biochemistry Molecular biology Takifugu In vivo Animals Protein Isoforms RNA Messenger Molecular Biology In Situ Hybridization DNA Primers Whole blood |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 148:225-230 |
| ISSN: | 1096-4959 |
| DOI: | 10.1016/j.cbpb.2007.02.016 |
| Popis: | In teleosts, two distinct types of TIMP-2s occur, TIMP-2a and TIMP-2b, but little is known about their locations and quantitative expressions. Here, we examined pufferfish (Takifugu rubripes) TIMP-2a (fgTIMP-2a) and TIMP-2b (fgTIMP-2b) quantities and locations in fugu adult tissues and embryos. To compare the quantitative expression of fgTIMP-2s, we performed a quantitative real-time PCR (qPCR). FgTIMP-2a mRNA was constitutively expressed and significant differences in expression were not observed among adult tissues. Whereas, fgTIMP-2b mRNA was significantly differently expressed in ordinary muscle and gill compared to the expression level in whole blood (P |
| Databáze: | OpenAIRE |
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