Oropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery
Autor: | Liang Zhou, Hui-Ying Huang, Ming Zhang, Ji Sun, Hui-Ching Lau, Chi-Yao Hsueh, Hongli Gong |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Microbiology (medical)
Adult Male medicine.medical_specialty medicine.medical_treatment Veillonella Oropharynx Antineoplastic Agents medicine.disease_cause Microbiology Hypopharyngeal Carcinoma stomatognathic system medicine Humans Induced chemotherapy Phylogeny Aged Chemotherapy biology Bacteria Streptococcus Microbiota Research Carcinoma Pharyngeal Neoplasms Hypopharyngeal carcinoma Middle Aged Fusobacterium biology.organism_classification medicine.disease QR1-502 Surgery 16S rRNA sequencing stomatognathic diseases Gemella Female Dysbiosis Actinomyces |
Zdroj: | BMC Microbiology, Vol 21, Iss 1, Pp 1-13 (2021) BMC Microbiology |
ISSN: | 1471-2180 |
Popis: | Aims To analyze changes in oropharynx microbiota composition after receiving induced chemotherapy followed by surgery for hypopharyngeal squamous cell carcinoma (HPSCC) patients. Methods Clinical data and swab samples of 38 HPSCC patients (HPSCC group) and 30 patients with benign disease (control group, CG) were enrolled in the study. HPSCC group was stratified into two groups: induced chemotherapy group (IC) of 10 patients and non-induced chemotherapy group (nIC) of 28 patients. The microbiota from oropharyngeal membrane was analyzed through 16S rRNA sequencing. Results Alpha-diversity (Shannon and Ace indexes) and weighted UniFrac based beta-diversity severely decreased in the HPSCC group when compared with CG. In pre-operative comparisons, PCoA and NMDS analyses showed microbial structures in the IC group were more similar to CG than nIC. Both IC group and nIC group yielded significantly diverse post-operative communities in contrast to their pre-operative counterparts, evident by the decrease in genera Veillonella and Fusobacterium and increase in genera Streptococcus and Gemella. Given that post-operative oropharynx microbiota showed no difference between IC and nIC groups, the IC group showed less accumulation in anaerobic communities. The abundance of genera Fusobacterium, Parvimonas, Actinomyces were enhanced in the advanced stages (III/IV). Conclusions Oropharynx microbiota in the HPSCC group presents dysbiosis with low diversity and abundance. Induced chemotherapy is beneficial in adjusting the oropharynx microbial environment leading to fewer amounts of anaerobe accumulation after operation. Higher amounts of Fusobacterium in advanced stages (III/IV) may influence the progression of HPSCC. |
Databáze: | OpenAIRE |
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