Apoptosis and cell proliferation in porcine placental vascularization
| Autor: | Mariana Rita Fiorimanti, Eva Gabriela Sanchis, Andrea Lorena Cristofolini, Cecilia Inés Merkis, Claudio Gustavo Barbeito |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Angiogenesis Swine Otras Ciencias Biológicas Placenta Cell Neovascularization Physiologic Apoptosis Biology Andrology Ciencias Biológicas 03 medical and health sciences Endocrinology ULTRASTRUCTURE Food Animals vascularization Pregnancy Otras Ciencias Veterinarias medicine Animals PIGS PLACENTA VASCULARIZATION Cell Proliferation Fetus TUNEL assay Cell growth Ciencias Veterinarias pigs General Medicine ultrastructure Placentation APOPTOSIS 030104 developmental biology medicine.anatomical_structure CIENCIAS AGRÍCOLAS Gestation Animal Science and Zoology Female Veterinaria CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| Popis: | The placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30 ± 2(n =5), 40 ± 2(n =5), 60 ± 2 (n =5), 80 ± 2(n =5), 90 ± 2(n =5) and 114 ± 2(n =5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30 ± 2 and 80 ± 2 of pregnancy. Caspase-3 expressed the highest value on day 30 ± 2, while the highest apoptotic indexes were found on days30 ± 2 and 90 ± 2. The placental vascular area was higher on day 80 ± 2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80 ± 2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation. Facultad de Ciencias Veterinarias |
| Databáze: | OpenAIRE |
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