A plant genetically modified that accumulates Pb is especially promising for phytoremediation
| Autor: | Antonio De Haro, Carmina Gisbert, David J. Walker, Juan Navarro-Aviñó, Ramón Serrano, M. Pilar Bernal, Roc Ros |
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| Přispěvatelé: | Cooperativa Agrícola del Puzol, Caja Rural de Valencia, European Commission, Ministerio de Ciencia y Tecnología (España) |
| Rok vydání: | 2003 |
| Předmět: |
Agrobacterium
Biophysics Plant Roots Biochemistry Hyperaccumulators Bioremediation Metals Heavy Tobacco Botany Nicotiana glauca Hyperaccumulator Molecular Biology Glucuronidase Nicotiana biology fungi Wild type food and beverages Biological Transport Cell Biology Plants Genetically Modified biology.organism_classification Recombinant Proteins Genetically modified organism Phytoremediation Biodegradation Environmental Lead Seedling Phytochelatin synthase Cadmium |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 6 pages, 3 figures, 1 table. From a number of wild plant species growing on soils highly contaminated by heavy metals in Eastern Spain, Nicotiana glauca R. Graham (shrub tobacco) was selected for biotechnological modification, because it showed the most appropriate properties for phytoremediation. This plant has a wide geographic distribution, is fast-growing with a high biomass, and is repulsive to herbivores. Following Agrobacterium mediated transformation, the induction and overexpression of a wheat gene encoding phytochelatin synthase (TaPCS1) in this particular plant greatly increased its tolerance to metals such as Pb and Cd, developing seedling roots 160% longer than wild type plants. In addition, seedlings of transformed plants grown in mining soils containing high levels of Pb (1572 ppm) accumulated double concentration of this heavy metal than wild type. These results indicate that the transformed N. glauca represents a highly promising new tool for use in phytoremediation efforts. This work has been carried out in collaboration with Spanish private companies COPUZOL and CAJA RURAL VALENCIA. Funds were provided by the European Union (F.E.D.E.R., 1FD97-1469-C04-01) and Spanish Ministry of Science and Technology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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