Low prevalence of hypervirulent Klebsiella pneumoniae in Anatolia, screened via phenotypic and genotypic testing
| Autor: | Selay Demirci-Duarte, Aslı Çakar, Banu Sancak, Cumhur Özkuyumcu, Tugce Unalan-Altintop, Koray Ergünay, Serra Orsten |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Adolescent Turkey Virulence Factors medicine.drug_class Klebsiella pneumoniae Antibiotics Microbial Sensitivity Tests Biology Tertiary care Microbiology Tertiary Care Centers Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Bacterial Proteins Drug Resistance Multiple Bacterial Genotype medicine Humans Blood culture 030212 general & internal medicine Child Aged 0303 health sciences Virulence General Immunology and Microbiology medicine.diagnostic_test 030306 microbiology String test Infant General Medicine Middle Aged biology.organism_classification Phenotype Anti-Bacterial Agents Klebsiella Infections chemistry Child Preschool Aerobactin Female |
| Zdroj: | Acta Microbiologica et Immunologica Hungarica. 67:120-126 |
| ISSN: | 1588-2640 1217-8950 |
| DOI: | 10.1556/030.2020.01143 |
| Popis: | Hypervirulent Klebsiella pneumoniae (hvKP) strains are associated with vigorous clinical presentation and relapses. Initially reported from Asia, these variants have spread globally and become an emerging agent of significant health threat. This study was carried out to identify hvKP strains in a previously uninvestigated region and to evaluate the impact of commonly-employed phenotypic and genotypic markers as diagnostic assays. A total of 111 blood culture isolates, collected at a tertiary care center was investigated. The hvKP strains were sought by a string test and the amplification of partial magA, rmpA, iucA and peg344. All products were characterized via sequencing. Evidence for hvKP was observed in 10.8% via iucA amplification (7.2%), string test (2.7%) and magA amplification (0.9%). Specific products were not produced by assays targeting rmpA and peg344 genes. Antibiotic susceptibility patterns compatible with possible extensive or pan-antimicrobial resistance was noted in 66.7% of the hvKP candidate strains. Capsule type in the magA positive strain was characterized as K5. We have detected hvKP in low prevalence at a region with no prior documentation. Targetting the aerobactin gene via iucA amplification provided the most accurate detection in this setting. The epidemiology of hvKP in Anatolia requires elucidation for effective control and management. |
| Databáze: | OpenAIRE |
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