Evaluation of Capillary Zone Electrophoresis and Micellar Electrokinetic Capillary Chromatography with Direct Injection of Plasma for the Determination of Cefotaxime and Its Metabolite

Autor:	Fabre H, Castaneda Penalvo G, Mary T. Kelly, Maillols H
Rok vydání:	1997
Předmět:	Detection limit Chromatography Analyte Chemistry Metabolite Electrophoresis Capillary Cefotaxime Micellar electrokinetic chromatography Analytical Chemistry Dilution chemistry.chemical_compound Capillary electrophoresis Humans Quantitative analysis (chemistry) Micelles Antibacterial agent
Zdroj:	Analytical Chemistry. 69:1364-1369
ISSN:	1520-6882 0003-2700
DOI:	10.1021/ac9605049
Popis:	Quantitative aspects of capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MECC) were investigated for the determination of cefotaxime (C) and its deacetyl metabolite (DA) in human plasma in a concentration range of therapeutic interest. For CZE, plasma samples spiked with C and DA were injected after deproteinization with acetonitrile, and analytes were separated in a fused silica capillary using a borate buffer at pH 9.2 as electrolyte ; no suitable internal standard was found. For MECC, plasma samples spiked with C, DA, and theobromine as internal standard were directly injected after dilution with water and analyzed using a phosphate buffer, pH 8.00, containing 165 mM SDS as separation electrolyte and a fused silica capillary. Both methods gave satisfactory interday precision with respect to migration times (RSD
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f917a3edb949c224034480ac6db6c38f https://doi.org/10.1021/ac9605049 Zobrazit plný text záznamu