Characterization of Haemophilus parasuis Serovar 2 CL120103, a Moderately Virulent Strain in China
| Autor: | Wang Chenyan, Che Yongliang, Chen Rujing, Lun-jiang Zhou, Wu Xuemin, Long-bai Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
QH301-705.5 comprehensive genetic analysis Virulence Genome General Biochemistry Genetics and Molecular Biology DNA sequencing 03 medical and health sciences virulence-associated gene lps biosynthesis Biology (General) Actinobacillus pleuropneumoniae Gene Genetics General Immunology and Microbiology biology General Neuroscience Ribosomal RNA biology.organism_classification genome sequencing virulence 030104 developmental biology haemophilus parasuis RRNA Operon General Agricultural and Biological Sciences GC-content |
| Zdroj: | Open Life Sciences, Vol 13, Iss 1, Pp 217-226 (2018) |
| ISSN: | 2391-5412 |
| Popis: | Haemophilus parasuisis an important bacterium affecting pigs, causing Glässer’s disease. To further characterize this species, we determined the complete genomic sequence ofH. parasuisCL120103, which was isolated from diseased pigs. The strainH. parasuisCL120103 was identified as serovar 2. The size of the largest scaffold is 2,326,318 bp and contains 145 large contigs, with the N50 contig being 20,573 bp in length. The complete genome ofH. parasuisCL120103 is 2,305,354 bp in length with 39.97% GC content and contains 2227 protein-coding genes, 19 ribosomal rRNA operons and 60 tRNA genes. Sequence similarity of the genome ofH. parasuisCL120103 to the previously sequenced genome ofH. parasuiswas up to 96% and query cover to 86%. Annotation of the genome ofH. parasuisCL120103 identified a number of genes encoding potential virulence factors. These virulence factors are involved in metabolism, adhesion, secretion and LPS biosynthesis. These related genes pave the way to better understand mechanisms underlying metabolic capabilities. The comprehensive genetic and phylogenetic analysis shows thatH. parasuisis closely related toActinobacillus pleuropneumoniaeand provides a foundation for future experimental confirmation of the virulence and pathogen-host interactions inH. parasuis. |
| Databáze: | OpenAIRE |
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