The Physical Chemistry of the Enigmatic Phospholipid Diacylglycerol Pyrophosphate
| Autor: | Liza Strawn, Amy Babb, Christa Testerink, Edgar E. Kooijman |
|---|---|
| Přispěvatelé: | Plant Physiology (SILS, FNWI), Green Life Sciences |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Effective lipid shape Ionization effective lipid shape Phospholipid Plant Science lcsh:Plant culture electrostatic hydrogen bond switch dgpp 01 natural sciences diacylglycerolpyrophosphate 03 medical and health sciences chemistry.chemical_compound Phosphatidylcholine Phosphatidic acid ionization Lipid second messenger lcsh:SB1-1110 Diacylglycerol pyrophosphate Lipid bilayer diacylglycerol pyrophosphate Dgpp Original Research 030304 developmental biology Phosphatidylethanolamine 0303 health sciences Bilayer phospholipid signaling phosphatidic acid Phospholipid signaling lipid second messenger chemistry Biochemistry Electrostatic hydrogen bond switch Second messenger system Biophysics 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 3 (2012) Frontiers in Plant Science 3 (2012) Frontiers in plant science Frontiers in Plant Science, 3 Frontiers in Plant Science, 3. Frontiers Media S.A. |
| ISSN: | 1664-462X |
| Popis: | Phosphatidic acid (PA) is a lipid second messenger that is formed transiently in plants in response to different stress conditions, and plays a role in recruiting protein targets, ultimately enabling an adequate response. Intriguingly, this increase in PA concentration in plants is generally followed by an increase in the phospholipid diacylglycerolpyrophosphate (DGPP), via turnover of PA. Although DGPP has been shown to induce stress-related responses in plants, it is unclear to date what its molecular function is and how it exerts its effect. Here, we describe the physicochemical properties, i.e., effective molecular shape and charge, of DGPP. We find that unlike PA, which imparts a negative curvature stress to a (phospho)lipid bilayer, DGPP stabilizes the bilayer phase of phosphatidylethanolamine (PE), similar to the effect of phosphatidylcholine (PC). DGPP thus has zero curvature. The pKa(2) of the phosphomonoester of DGPP is 7.44 +/- 0.02 in a PC bilayer, compared to a pKa(2) of 7.9 for PA. Replacement of half of the PC with PE decreases the pKa(2) of DGPP to 6.71 +/- 0.02, similar to the behavior previously described for PA and summarized in the electrostatic-hydrogen bond switch model. Implications for the potential function of DGPP in biomembranes are discussed. |
| Databáze: | OpenAIRE |
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