Reduced growth of Listeria monocytogenes in two model cheese microcosms is not associated with individual microbial strains
| Autor: | Marielle Guéguen, Jean-Michel Bré, Nathalie Desmasures, Muhammad Imran, Jean-Paul Vernoux |
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| Přispěvatelé: | Quaid-i-Azam University (QAU), Aliments Bioprocédés Toxicologie Environnements (ABTE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Département Matériaux (IFSTTAR/MAT), Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-PRES Université Paris-Est |
| Rok vydání: | 2013 |
| Předmět: |
BACTERIOLOGIE
Population Down-Regulation Geotrichum BACTERIE medicine.disease_cause Serratia liquefaciens Microbiology 03 medical and health sciences Paenibacillus RESEAU D'ASSAINISSEMENT Species Specificity Listeria monocytogenes Cheese medicine education 030304 developmental biology 2. Zero hunger 0303 health sciences education.field_of_study Bacteria biology 030306 microbiology MICROORGANISME Hydrogen-Ion Concentration biology.organism_classification Staphylococcus equorum CONTAMINATION Kinetics [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology MICROBIOLOGIE Listeria Microcosm Food Science |
| Zdroj: | Food Microbiology Food Microbiology, Elsevier, 2013, 33 (1), pp 30-39. ⟨10.1016/j.fm.2012.08.008⟩ |
| ISSN: | 0740-0020 1095-9998 |
| DOI: | 10.1016/j.fm.2012.08.008 |
| Popis: | Two model antilisterial microbial communities consisting of two yeasts, two Gram positive and two Gram negative bacteria, and originating from Livarot cheese smear were previously designed. They were used in the present study to analyse the impact of microbial population dynamics on growth of Listeria monocytogenes in cheese microcosm. Specific culture media and PCR primers were developed for simultaneous culture-dependent and real-time PCR quantification of strains belonging to Marinomonas sp., Paenibacillus sp., Staphylococcus equorum , Arthrobacter arilaitensis , Pseudomonas putida , Serratia liquefaciens , Candida natalensis , and Geotrichum candidum , in cheese microcosms. All strains were enumerated after 3, 5, 8 and 14 days at 15 °C. They established well at high counts in all cheese microcosms. Growth dynamics for all strains in presence of L. monocytogenes WSLC 1685 were compared to those of microbial communities obtained by omitting in turn one of the six members of the initial community. The growth of the microbial strains was neither markedly disturbed by Listeria presence nor by the removal of each strain in turn. Furthermore, these communities had a significant reducing effect on growth of L. monocytogenes independently of pH, as confirmed by mathematical modelling. A barrier effect was observed, that could be explained by specific competition for nutrients. |
| Databáze: | OpenAIRE |
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