A nitrocellulose membrane-based integrated microfluidic system for bacterial detection utilizing magnetic-composite membrane microdevices and bacteria-specific aptamers
| Autor: | Chih-Hung Wang, Yu-Dong Ma, Jia-Han Wu, Gwo-Bin Lee |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Magnetic composite Aptamer Microfluidics Biomedical Engineering Bioengineering Nanotechnology 02 engineering and technology 01 natural sciences Biochemistry chemistry.chemical_compound Limit of Detection Lab-On-A-Chip Devices Collodion Detection limit Bacteria biology 010401 analytical chemistry Membranes Artificial Equipment Design General Chemistry Aptamers Nucleotide Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Membrane chemistry Colorimetry 0210 nano-technology Nitrocellulose |
| Zdroj: | Lab on a Chip. 18:1633-1640 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c8lc00251g |
| Popis: | Bacteria such as Acinetobacter baumannii (AB) can cause serious infections, resulting in high mortality if not diagnosed early and treated properly; there is consequently a need for rapid and accurate detection of this bacterial species. Therefore, we developed a new, nitrocellulose-based microfluidic system featuring AB-specific aptamers capable of automating the bacterial detection process via the activity of microfluidic devices composed of magnetic-composite membranes. Electromagnets were used to actuate these microfluidic devices such that the entire diagnostic process could be conducted in the integrated microfluidic system within 40 minutes with a limit of detection as low as 450 CFU per reaction for AB. Aptamers were used to capture AB in complex samples on nitrocellulose membranes, and a simple colorimetric assay was used to estimate bacterial loads. Given the ease of use, portability, and sensitivity of this aptamer-based microfluidic system, it may hold great promise for point-of-care diagnostics. |
| Databáze: | OpenAIRE |
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