EVIDENCE THAT DRUG-RESISTANT ALLOREACTIVE T CELLS MAY CONTRIBUTE TO HUMAN GRAFT REJECTION
| Autor: | Gary H. S. Strauss, William L. Stanford, Olivera J. Finn |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Graft Rejection
Transplantation Chemotherapy business.industry T-Lymphocytes medicine.medical_treatment T cell Drug Resistance Purine analogue Azathioprine T lymphocyte Drug resistance In vitro Cell Line medicine.anatomical_structure Cell culture Immunology Cancer research Humans Medicine Thioguanine business Cell Division medicine.drug |
| Zdroj: | Transplantation. 49:1138-1141 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/00007890-199006000-00022 |
| Popis: | The objective of our study was to determine whether resistance to immunosuppressive drugs by transplant recipient's T cells could contribute to continued graft rejection, in spite of immunosuppressive therapy. The T cell lines used in this series of experiments were originally established from T cells that had infiltrated kidney or liver grafts and initiated rejections in patients receiving immunosuppressive drugs, including the purine analogue azathioprine (AZ). We have used a proliferation assay and the Strauss-Albertini test to analyze the T cell lines. Both assays use 6-thioguanine (6-TG), an amino derivative of AZ, as the selective agent to measure the resistance to AZ. Although the frequency of 6-TG-resistant variants in the majority of T cell lines closely resembled the frequency found in peripheral blood lymphocytes of controls, we identified four cell lines that demonstrated virtually complete resistance to the purine analogue 6-TG in both assays. These observations indicate that a more effective immunosuppressive treatment may be achieved by using a combination of drugs. |
| Databáze: | OpenAIRE |
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