The adaptive response to iron involves changes in energetic strategies in the pathogen Candida albicans

Autor: Duval, Celia, Macabiou, Carole, Garcia, Camille, Lesuisse, Emmanuel, Camadro, Jean-Michel, Auchère, Françoise, Camadro, Jean‐Michel
Přispěvatelé: Institut Jacques Monod (IJM (UMR_7592)), Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
MESH: Oxidation-Reduction
[SDV]Life Sciences [q-bio]
Adaptation
Biological
Respiratory chain
lcsh:QR1-502
MESH: Virulence
lcsh:Microbiology
Adenosine Triphosphate
iron
Tandem Mass Spectrometry
Candida albicans
MESH: Adenosine Triphosphate
oxidative stress
Pathogen
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
MESH: Glutathione
0303 health sciences
MESH: Iron
MESH: Oxidative Stress
Virulence
biology
MESH: Energy Metabolism
Candidiasis
MESH: Reactive Oxygen Species
Iron deficiency
Glutathione
Corpus albicans
MESH: Candidiasis
filamentation
mitochondria
Original Article
MESH: Fungal Proteins
Oxidation-Reduction
Metabolic Networks and Pathways
Hypha
MESH: Mitochondria
MESH: Biological Transport
Microbiology
Fungal Proteins
03 medical and health sciences
medicine
030304 developmental biology
030306 microbiology
MESH: Adaptation
Biological
MESH: Candida albicans
MESH: Tandem Mass Spectrometry
Biological Transport
Original Articles
medicine.disease
biology.organism_classification
Yeast
MESH: Metabolic Networks and Pathways
Energy Metabolism
Reactive Oxygen Species
metabolism
MESH: Chromatography
Liquid
Chromatography
Liquid
Zdroj: MicrobiologyOpen
MicrobiologyOpen, Wiley, 2019, 9 (2), pp.e970. ⟨10.1002/mbo3.970⟩
MicrobiologyOpen, Vol 9, Iss 2, Pp n/a-n/a (2020)
MicrobiologyOpen, Wiley, In press, pp.e970. ⟨10.1002/mbo3.970⟩
ISSN: 2045-8827
DOI: 10.1002/mbo3.970⟩
Popis: Candida albicans is an opportunist pathogen responsible for a large spectrum of infections, from superficial mycosis to systemic diseases known as candidiasis. Its ability to grow in different morphological forms, such as yeasts or filamentous hyphae, contributes to its survival in diverse microenvironments. Iron uptake has been associated with virulence, and C. albicans has developed elaborate strategies for acquiring iron from its host. In this work, we analyze the metabolic changes in response to changes in iron content in the growth medium and compare C. albicans adaptation to the presence or absence of iron. Functional and morphological studies, correlated to a quantitative proteomic analysis, were performed to assess the specific pathways underlying the response to iron, both in the yeast and filamentous forms. Overall, the results show that the adaptive response to iron is associated with a metabolic remodeling affecting the energetic pathways of the pathogen. This includes changes in the thiol‐dependent redox status, the activity of key mitochondrial enzymes and the respiratory chain. Iron deficiency stimulates bioenergetic pathways, whereas iron‐rich condition is associated with greater biosynthetic needs, particularly in filamentous forms. Moreover, we found that C. albicans yeast cells have an extraordinary capability to adapt to changes in environmental conditions.
We analyze here the adaptive response of Candida albicans cells to changes in the iron content of the culture environment. This response includes changes in intracellular redox status and the reorientation of metabolic pathways, as shown by label‐free analyses and biochemical measurements. We found that iron deficiency stimulated the TCA cycle, mitochondrial respiratory chain, and ATP production, to compensate for cellular stress, to maintain normal levels of ATP, and to ensure cell survival. Conversely, an increase of iron is associated with biosynthetic needs, especially in filamentous forms.
Databáze: OpenAIRE
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