Development and characterization of a microfluidic model of the tumour microenvironment
| Autor: | Manuel Doblaré, Ignacio Ochoa, Simon J. Allison, Eduardo Fernández, Julián Pardo, Pablo Botella, Jose M. Ayuso, Alodia Lacueva, María Virumbrales-Muñoz, Elisa Checa-Chavarria, Pilar M. Lanuza, Luis J. Fernández, Roger M. Phillips |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Survival Microfluidics Cell Cell Culture Techniques Antineoplastic Agents Apoptosis Biology Q1 Time-Lapse Imaging Article QH301 03 medical and health sciences Cell Line Tumor Spheroids Cellular Tumor Microenvironment medicine Humans Cell Proliferation Tumor microenvironment Multidisciplinary Cell growth Spheroid Hydrogels HCT116 Cells In vitro Cell biology Killer Cells Natural Oxygen Glucose 030104 developmental biology medicine.anatomical_structure Microscopy Fluorescence Doxorubicin Cell culture Immunology Cancer cell Reactive Oxygen Species |
| Zdroj: | Zaguán. Repositorio Digital de la Universidad de Zaragoza instname Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep36086 |
| Popis: | The physical microenvironment of tumours is characterized by heterotypic cell interactions and physiological gradients of nutrients, waste products and oxygen. This tumour microenvironment has a major impact on the biology of cancer cells and their response to chemotherapeutic agents. Despite this, most in vitro cancer research still relies primarily on cells grown in 2D and in isolation in nutrient- and oxygen-rich conditions. Here, a microfluidic device is presented that is easy to use and enables modelling and study of the tumour microenvironment in real-time. The versatility of this microfluidic platform allows for different aspects of the microenvironment to be monitored and dissected. This is exemplified here by real-time profiling of oxygen and glucose concentrations inside the device as well as effects on cell proliferation and growth, ROS generation and apoptosis. Heterotypic cell interactions were also studied. The device provides a live ‘window’ into the microenvironment and could be used to study cancer cells for which it is difficult to generate tumour spheroids. Another major application of the device is the study of effects of the microenvironment on cellular drug responses. Some data is presented for this indicating the device’s potential to enable more physiological in vitro drug screening. |
| Databáze: | OpenAIRE |
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