Pam16 has an essential role in the mitochondrial protein import motor
| Autor: | Albert Sickmann, Virginia Bilanchone, M G Cumsky, Kaye N. Truscott, Helmut E. Meyer, Yanfeng Li, Andreas Geissler, Ann E. Frazier, Wolfgang Voos, Chris Meisinger, Maria I. Lind, Peter Rehling, Jan Dudek, Bernard Guiard, Nikolaus Pfanner |
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| Rok vydání: | 2004 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Translocase of the outer membrane Protein subunit Mitochondrial Membrane Transport Proteins Mitochondrial Proteins Mitochondrial membrane transport protein Structural Biology Yeasts Mitochondrial Precursor Protein Import Complex Proteins Translocase Inner membrane HSP70 Heat-Shock Proteins Protein Precursors Molecular Biology biology Membrane transport protein Molecular Motor Proteins Membrane Proteins Membrane Transport Proteins Intracellular Membranes Mitochondria Cell biology Transport protein Protein Transport Translocase of the inner membrane biology.protein Carrier Proteins Protein Binding |
| Zdroj: | Nature Structural & Molecular Biology. 11:226-233 |
| ISSN: | 1545-9985 1545-9993 |
| DOI: | 10.1038/nsmb735 |
| Popis: | Mitochondrial preproteins destined for the matrix are translocated by two channel-forming transport machineries, the translocase of the outer membrane and the presequence translocase of the inner membrane. The presequence translocase-associated protein import motor (PAM) contains four essential subunits: the matrix heat shock protein 70 (mtHsp70) and its three cochaperones Mge1, Tim44 and Pam18. Here we report that the PAM contains a fifth essential subunit, Pam16 (encoded by Saccharomyces cerevisiae YJL104W), which is selectively required for preprotein translocation into the matrix, but not for protein insertion into the inner membrane. Pam16 interacts with Pam18 and is needed for the association of Pam18 with the presequence translocase and for formation of a mtHsp70-Tim44 complex. Thus, Pam16 is a newly identified type of motor subunit and is required to promote a functional PAM reaction cycle, thereby driving preprotein import into the matrix. |
| Databáze: | OpenAIRE |
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