Evaluation of the FilmArray Blood Culture Identification Panel on Detection of Pathogenic Microorganisms in Positive Blood Cultures: the First Clinical Report in Japan
Autor: | Chiyuki Ishizawa, Mitsuo Kaku, Shinya Inomata, Shiro Endo, Makoto Katsumi, Kyoichi Saito, Masahiro Toyokawa, Yuko Fujikawa |
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Rok vydání: | 2018 |
Předmět: |
Adult
DNA Bacterial Male 0301 basic medicine Microbiology (medical) Klebsiella pneumoniae Microorganism Genes Fungal 030106 microbiology Microbiology Sepsis Young Adult 03 medical and health sciences Antibiotic resistance Japan Drug Resistance Bacterial Multiplex polymerase chain reaction medicine Humans Blood culture DNA Fungal Aged Retrospective Studies Aged 80 and over Bacteria medicine.diagnostic_test biology Fungi General Medicine Middle Aged biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification Yeast Molecular Typing Infectious Diseases Blood Culture Genes Bacterial Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Female Multiplex Polymerase Chain Reaction |
Zdroj: | Japanese Journal of Infectious Diseases. 71:145-147 |
ISSN: | 1884-2836 1344-6304 |
Popis: | FilmArray (FA) is a multiplex PCR-based desktop microbial detection system. The blood culture identification (BCID) panel is an adaptable panel for FA, which diagnoses sepsis and/or systemic infections by detecting 14 bacterial species, 4 bacterial genera, 1 bacterial family, 5 yeast species, and 3 antimicrobial resistance genes (mecA, Klebsiella pneumoniae carbapenemase [KPC], and vanA/B) in positive blood cultures within 1 h. We retrospectively evaluated the FA-BCID panel using 54 positive blood cultures, in which 57 bacterial and 3 yeast strains were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The FA-BCID panel revealed 59 microorganisms in 53 samples; this performance was similar to that of MALDI-TOF MS analysis; however, 1 bacterium in 1 sample was not detected. In addition, mecA genes were detected in 12 Staphylococcus species, which all manifested methicillin resistance in susceptibility testing, whereas genes KPC and vanA/B were not detected, in agreement with the results of antimicrobial susceptibility testing. Although more information on antimicrobial resistance, including activity of IMP-metallo-β-lactamases, is required in Japan, the FA-BCID panel can detect pathogenic microorganisms in positive blood cultures rapidly, and this method could be beneficial for proper treatment of sepsis and/or systemic infections, especially in small hospitals. |
Databáze: | OpenAIRE |
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