Insights Into Vaginal Bacterial Communities and Metabolic Profiles of Chlamydia trachomatis Infection: Positioning Between Eubiosis and Dysbiosis
| Autor: | Melissa Salvo, Carola Eleonora Parolin, Luca Laghi, Antonietta D'Antuono, Nicoletta Banzola, Antonella Marangoni, Roberto Cevenini, Barbara Giordani, Clarissa Consolandi, Marco Severgnini, Chenglin Zhu, Claudio Foschi, Beatrice Vitali, Valeria Gaspari |
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| Přispěvatelé: | Carola Parolin, Claudio Foschi, Luca Laghi, Chenglin Zhu, Nicoletta Banzola, Valeria Gaspari, Antonietta D’Antuono, Barbara Giordani, Marco Severgnini, Clarissa Consolandi, Melissa Salvo, Roberto Cevenini, Beatrice Vitali, Antonella Marangoni |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology lcsh:QR1-502 Chlamydia trachomatis medicine.disease_cause Microbiology lcsh:Microbiology 03 medical and health sciences Lactobacillus iners medicine Metabolome Gardnerella vaginalis Microbiome Vaginal metabolome Original Research biology Lactobacillus crispatus business.industry Bacterial vaginosis biology.organism_classification medicine.disease Chlamydia trachomatis eubiosis bacterial vaginosis vaginal microbiota vaginal metabolome 030104 developmental biology Vaginal microbiota business Eubiosis Dysbiosis |
| Zdroj: | Frontiers in microbiology 9 (2018). doi:10.3389/fmicb.2018.00600 info:cnr-pdr/source/autori:Parolin C.; Foschi C.; Laghi L.; Zhu C.; Banzola N.; Gaspari V.; D'Antuono A.; Giordani B.; Severgnini M.; Consolandi C.; Salvo M.; Cevenini R.; Vitali B.; Marangoni A./titolo:Insights into vaginal bacterial communities and metabolic profiles of Chlamydia trachomatis infection: Positioning between eubiosis and dysbiosis/doi:10.3389%2Ffmicb.2018.00600/rivista:Frontiers in microbiology/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume:9 Frontiers in Microbiology, Vol 9 (2018) Frontiers in Microbiology |
| Popis: | The vaginal microbiota plays a crucial role in maintaining the health and functioning of the female genital tract, preventing the colonization of urogenital pathogens and sexually transmitted infections. In this study, we characterized the vaginal bacterial communities and the metabolome associated to Chlamydia trachomatis infection (CT: 20 women), compared to healthy condition (H: 22 women) and bacterial vaginosis (BV: 19 women). A microarray-based tool (VaginArray), implemented with a real-time PCR for Gardnerella vaginalis, was used to determine the vaginal bacterial composition, whereas the metabolic profiles were assessed by a proton-based nuclear magnetic resonance (1H-NMR) spectroscopy. CT infection was characterized by bacterial and metabolic signatures similar to healthy condition, even though higher amounts of Lactobacillus iners, as well as depletion of some amino acids, biogenic amines, and succinate marked CT infection. Moreover, the frequency of Lactobacillus crispatus was higher in asymptomatic CT-positive patients than in women with CT-correlated symptoms. We also confirmed the marked differences in the microbiome and metabolome between healthy and BV-affected women. In conclusion, we highlighted microbial and metabolic peculiarities of the vaginal ecosystem in the case of CT infection, even though further studies are needed to understand if the observed alterations precede the infection onset or if the pathogen itself perturbs the vaginal environment. |
| Databáze: | OpenAIRE |
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