Differential modulation of BMP signaling promotes the elaboration of cerebral cortical GABAergic neurons or oligodendrocytes from a common sonic hedgehog-responsive ventral forebrain progenitor species

Autor: Joseph J. Abrajano, Mark F. Mehler, Shau Yu Yung, Aldrin E. Molero, Solen Gokhan, Jennifer Jurcsak
Rok vydání: 2002
Předmět:
Recombinant Fusion Proteins
Oligodendrocyte Transcription Factor 2
Green Fluorescent Proteins
Models
Neurological

Bone Morphogenetic Protein 2
Nerve Tissue Proteins
Bone morphogenetic protein
Oligodeoxyribonucleotides
Antisense

Mice
Prosencephalon
Genes
Reporter

Transforming Growth Factor beta
Basic Helix-Loop-Helix Transcription Factors
medicine
Animals
Cell Lineage
Hedgehog Proteins
Sonic hedgehog
Cells
Cultured

gamma-Aminobutyric Acid
Cerebral Cortex
Homeodomain Proteins
Mice
Knockout

Neurons
Multidisciplinary
biology
Genes
Homeobox

Gene Expression Regulation
Developmental

Biological Sciences
Molecular biology
Neural stem cell
DNA-Binding Proteins
Luminescent Proteins
Oligodendroglia
medicine.anatomical_structure
nervous system
Cerebral cortex
Bone Morphogenetic Proteins
Forebrain
Trans-Activators
biology.protein
GABAergic
Stem cell
Neuroscience
Transcription Factors
Zdroj: Proceedings of the National Academy of Sciences. 99:16273-16278
ISSN: 1091-6490
0027-8424
DOI: 10.1073/pnas.232586699
Popis: During cerebral cortical development, excitatory glutamatergic projection neurons are generated from neural stem cells intrinsic to the early embryonic cortical ventricular zone by a process of radial migration, whereas most inhibitory γ-aminobutyric acid (GABA)ergic interneurons and oligodendrocytes (OLs) appear to be elaborated from ventral forebrain stem cells that initially undergo tangential cortical migration before terminal lineage maturation. In contrast to the more compartmentalized developmental organization of the spinal cord, the generation of neurons and OLs from a common ventral forebrain stem cell would expose these cells to the sequential actions of ventral and dorsal gradient morphogens [sonic hedgehog (Shh) and bone morphogenetic proteins (BMPs)] that normally mediate opposing developmental programs. Here we report that Shh promotes GABAergic neuronal/OL lineage restriction of forebrain stem cells, in part, by activation of the basic helix–loop–helix transcription factors, Olig2 and Mash1. In mutant mice with a generalized defect in tangential cortical migration (Dlx1/2−/−), there is a profound and selective reduction in the elaboration of both cortical GABAergic neurons and OLs. Our studies further demonstrate that the sequential elaboration of cortical GABAergic neurons and OLs from common Shh-responsive ventral forebrain progenitors requires the spatial and temporal modulation of cortical BMP signaling by BMP ligands and the BMP antagonist, noggin, respectively. These findings suggest an integrative model for cerebral cortical GABAergic neuronal and OL lineage maturation that would incorporate the sequential contributions of the ventral and dorsal forebrain, and the potential role of regional developmental cues in modulating transcriptional codes within evolving neural lineage species.
Databáze: OpenAIRE