Characterization of the Cytotoxic Factor(s) Released from Thymic Dendritic Cells upon Human Immunodeficiency Virus Type 1 Infection
| Autor: | Isabelle Courchesne, Dominique Bergeron, Martin Richer, Éric A. Cohen, Sylvie Beaulieu, Marielle Lafontaine |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cytotoxicity
Immunologic Programmed cell death Apoptosis DNA Fragmentation Thymus Gland Cycloheximide Biology chemistry.chemical_compound Mice In vivo Virology Cytotoxic T cell Animals Humans fas Receptor Child Cells Cultured Protein Synthesis Inhibitors Tumor Necrosis Factor-alpha Cell Cycle Infant Newborn Virion Infant Proteins Dendritic Cells HLA-DR Antigens In vitro Killer Factors Yeast Cell biology Thymocyte chemistry Child Preschool Culture Media Conditioned Protein Biosynthesis HIV-1 Tumor necrosis factor alpha CD8 |
| Zdroj: | Virology. 241(2):285-297 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1997.8977 |
| Popis: | We previously demonstrated that infection of primary human thymic dendritic cells (DCs) with laboratory strains of HIV leads to the release of soluble factor(s) which induced thymocyte killing. In the present paper, we extend the characterization of this process. Our results reveal that primary HIV-1 isolates are similarly able to induce the production of cytotoxic factor(s) from thymic DCs and that the release of such factor(s) is dependent on viral infection. Interestingly, we observed that CD4 + and CD8 + purified thymocyte subsets, and activated PBMCs are susceptible to the cytotoxic activity, whereas freshly isolated resting PBMCs are resistant to this effect. Cycloheximide treatment prevents the killing of thymocytes exposed to HIV-infected DC supernatant, revealing that this form of cell death is an active biological process requiring protein synthesis. Finally, our data suggest that FasL and TNFα could both participate in the killing process. These in vitro observations provide a plausible model, whereby HIV-infected DCs can play a role in vivo in the induction of uninfected thymocyte killing. |
| Databáze: | OpenAIRE |
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