A method for the selective depletion of perivascular and meningeal macrophages in the central nervous system
| Autor: | Nico van Rooijen, Machteld M. J. Polfliet, Timo K. van den Berg, Peter H Goede, Esther M.L van Kesteren-Hendrikx, Christine D. Dijkstra |
|---|---|
| Přispěvatelé: | Other departments, Molecular cell biology and Immunology |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Central Nervous System
Male Pathology medicine.medical_specialty Immunology Central nervous system Population Cell Count Meninges parasitic diseases medicine Immunology and Allergy Macrophage Animals Rats Wistar education education.field_of_study Liposome Chemistry Macrophages Analgesics Non-Narcotic Rats Normal functioning Kinetics medicine.anatomical_structure Neurology Blood-Brain Barrier Liposomes Repopulation Neurology (clinical) Clodronic Acid |
| Zdroj: | Journal of neuroimmunology, 116(2), 188-195. Elsevier Journal of Neuroimmunology, 116(2), 188-95. Elsevier Polfliet, M M, Goede, P H, van Kesteren-Hendrikx, E M, van Rooijen, N, Dijkstra, C D & van den Berg, T K 2001, ' A method for the selective depletion of perivascular and meningeal macrophages in the central nervous system ', Journal of Neuroimmunology, vol. 116, no. 2, pp. 188-95 . |
| ISSN: | 0165-5728 |
| Popis: | The perivascular (PVM) and meningeal (MM) macrophages form a distinct population of resident CNS cells, selectively expressing the mature macrophage marker ED2 in the rat. In order to elucidate the role of the PVM and MM in rats during normal functioning of the brain and pathology, we have developed a strategy employing a single intraventricular injection of clodronate liposomes. This resulted in a complete depletion of the PVM and MM. Clodronate liposomes did not deplete the microglial cells. In other parts of the body, a temporal and mild depletion effect was observed, which was restored within 1 week. Detailed analysis of the elimination and repopulation kinetics of the PVM and MM revealed a slow repopulation of the CNS, starting at 14 days post depletion. This selective depletion method of the PVM and MM will enable us to get direct insight in their functions during normal and pathologic conditions of the CNS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect