Microbial expression systems for membrane proteins
| Autor: | Marvin V. Dilworth, Roslyn M. Bill, David R. Poyner, Stephane R. Gross, Peter J. F. Henderson, Bruno Miroux, Pikyee Ma, Karine Moncoq, Kim E. Bettaney, Mathilde S. Piel, Ji Luo, David Sharples |
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| Přispěvatelé: | Laboratoire de biologie physico-chimique des protéines membranaires (LBPC-PM (UMR_7099)), Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), ANR-11-LABX-0011,DYNAMO,Dynamique des membranes transductrices d'énergie : biogénèse et organisation supramoléculaire.(2011), Bettaney, K, Ma, P, Luo, J, Sharples, D, Poyner, D, Gross, S, Moncoq, K, Miroux, B, Bill, RM |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
[SDV.BIO]Life Sciences [q-bio]/Biotechnology [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] Bacteria Chemistry Membrane Proteins [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Promoter Computational biology General Biochemistry Genetics and Molecular Biology Recombinant Proteins law.invention 03 medical and health sciences 030104 developmental biology Membrane protein Cell culture law Solubilization Yeasts Recombinant DNA Promoter Regions Genetic Molecular Biology ComputingMilieux_MISCELLANEOUS Plasmids |
| Zdroj: | Methods Methods, Elsevier, 2018, 147, pp.3-39. ⟨10.1016/j.ymeth.2018.04.009⟩ |
| ISSN: | 1095-9130 1046-2023 |
| DOI: | 10.1016/j.ymeth.2018.04.009⟩ |
| Popis: | Despite many high-profile successes, recombinant membrane protein production remains a technical challenge; it is still the case that many fewer membrane protein structures have been published than those of soluble proteins. However, progress is being made because empirical methods have been developed to produce the required quantity and quality of these challenging targets. This review focuses on the microbial expression systems that are a key source of recombinant prokaryotic and eukaryotic membrane proteins for structural studies. We provide an overview of the host strains, tags and promoters that, in our experience, are most likely to yield protein suitable for structural and functional characterization. We also catalogue the detergents used for solubilization and crystallization studies of these proteins. Here, we emphasize a combination of practical methods, not necessarily high-throughput, which can be implemented in any laboratory equipped for recombinant DNA technology and microbial cell culture. |
| Databáze: | OpenAIRE |
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