Production and characterization of monoclonal antibodies against highly immunogenic Opisthorchis viverrini proteins and development of coproantigen detection
| Autor: | Yuji Arimatsu, Arpa Surapaitoon, Banchob Sripa, Salma Teimoori |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.drug_class
030231 tropical medicine Antibodies Helminth Enzyme-Linked Immunosorbent Assay Immunologic Tests Monoclonal antibody Opisthorchiasis 03 medical and health sciences 0302 clinical medicine Antigen Cell Line Tumor parasitic diseases medicine Parasite hosting Animals Humans Opisthorchis viverrini Molecular Biology 030304 developmental biology Immunoassay 0303 health sciences Hybridomas biology Opisthorchis Antibodies Monoclonal Helminth Proteins medicine.disease biology.organism_classification Virology Antigens Helminth Antibody Formation biology.protein Immunohistochemistry Parasitology Antibody Clone (B-cell biology) |
| Zdroj: | Molecular and biochemical parasitology. 240 |
| ISSN: | 1872-9428 |
| Popis: | Opisthorchis viverrini and other foodborne trematode infections are major health concerns in the Greater Mekong Subregion. Currently, the gold-standard diagnostic method for opisthorchiasis is conventional stool examination for the presence of parasite eggs. This method lacks sensitivity and needs an experienced technician. We therefore produced monoclonal antibodies to highly immunogenic O. viverrini proteins aiming at detecting specific antigens in the feces. In this study, BALB/C mice were immunized using semi-purified somatic antigens and spleen cells were fused with a Sp2/0 myeloma cell line. Four hybridomas (1A2, 1E12, 2C7 and 8D6) were selected and cloned due to their strong reaction against O. viverrini somatic protein, resulting in three IgM clones and one IgG2 clone. Immunohistochemistry showed that 1A2, 1E12, 2C7 and 8D6 stained the parenchyma cells, gut, tegument and muscles, respectively. Western-blot analysis revealed that only antibody 1A2 could detect coproantigen (approx. 73 kDa protein) in feces of hamsters infected with O. viverrini. The 1A2 monoclonal antibody may be of value in the diagnosis of opisthorchiasis by coproantigen detection. |
| Databáze: | OpenAIRE |
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