Metabolic Fingerprinting on a Plasmonic Gold Chip for Mass Spectrometry Based in Vitro Diagnostics
| Autor: | Deepanjali D. Gurav, Ruoping Chen, Vadanasundari Vedarethinam, Jiatao Lou, Xuming Sun, Jingjing Wan, Wei Xu, Ru Zhang, Jingyi Huang, Kun Qian, Lin Huang, Qian Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Materials science
General Chemical Engineering Nanotechnology 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology Mass spectrometry Chip 01 natural sciences 0104 chemical sciences Gold nanoshells Chemistry Laboratory automation Nanobiotechnology Ionization mass spectrometry 0210 nano-technology QD1-999 Plasmon Research Article |
| Zdroj: | ACS Central Science, Vol 4, Iss 2, Pp 223-229 (2018) ACS Central Science |
| ISSN: | 2374-7951 |
| Popis: | Current metabolic analysis is far from ideal to engage clinics and needs rationally designed materials and device. Here we developed a novel plasmonic chip for clinical metabolic fingerprinting. We first constructed a series of chips with gold nanoshells on the surface through controlled particle synthesis, dip-coating, and gold sputtering for mass production. We integrated the optimized chip with microarrays for laboratory automation and micro-/nanoscaled experiments, which afforded direct high-performance metabolic fingerprinting by laser desorption/ionization mass spectrometry using 500 nL of various biofluids and exosomes. Further we for the first time demonstrated on-chip in vitro metabolic diagnosis of early stage lung cancer patients using serum and exosomes. This work initiates a new bionanotechnology based platform for advanced metabolic analysis toward large-scale diagnostic use. A plasmonic chip was constructed for mass spectrometry based metabolic fingerprinting of biofluids and exosomes, toward large-scale diagnostic use. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|