Fast Patch-Based Pseudo-CT Synthesis from T1-Weighted MR Images for PET/MR Attenuation Correction in Brain Studies
| Autor: | Norberto Malpica, Eduardo Alcain, Juan Antonio Hernández-Tamames, Angel Torrado-Carvajal, Joaquin L. Herraiz, Lina Garcia-Cañamaque, Yves Rozenholc, Antonio S. Montemayor |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Time Factors Multimodal Imaging 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine Image Processing Computer-Assisted Medicine Humans Radiology Nuclear Medicine and imaging Stage (cooking) Sarcomatoid carcinoma Retrospective Studies Tumor microenvironment medicine.diagnostic_test business.industry Tumor-infiltrating lymphocytes Brain Magnetic resonance imaging Middle Aged medicine.disease Magnetic Resonance Imaging Positron emission tomography Positron-Emission Tomography Immunohistochemistry Female business Nuclear medicine Tomography X-Ray Computed 030217 neurology & neurosurgery CD8 Algorithms |
| Zdroj: | Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 57(1) |
| ISSN: | 1535-5667 |
| Popis: | 136 Objectives It is largely recognized that tumor microenvironment influences the efficacy of therapy and affects the outcome of different neoplasia. Therefore, we investigated the impact of metabolic information on FDG-PET in resectable non-small cell lung cancer (NSCLC), by comparing the imaging findings with the tissue expression of immune-checkpoints and other markers of tumor-related immunity. Methods All patients referred to our Institution for upfront surgical resection of NSCLC, who were investigated with FDG-PET prior to surgery (n=130) were consecutively enrolled in the study. From January 2010 to May 2014, 55 patients (stage IA-IIIB; M:F=42:13; mean age 68.9 years) were investigated. Sampled surgical tumor specimens were analyzed with immunohistochemistry (IHC) for CD68-TAMs (tumor-associated macrophages), CD8-TILs (tumor infiltrating Lymphocytes), PD-1 and PD-L1. The immunoreactivity was quantitatively evaluated and the scores compared with imaging findings. FDG-PET images were visualized on a GE Adw4.6 workstation and used to define semi-quantitative parameters (cut-off threshold 42%): i.e. SUVmax and SUVmean. Metabolic information on FDG-PET were correlated with tissue markers expression, and both with disease-free survival (DFS) considering a median follow-up of 16.2 months. Results Overall, 36 adenocarcinomas (ADK), 18 squamous cell carcinomas (SCCs), and 1 sarcomatoid carcinoma were analyzed. All investigated tumors resulted positive at FDG-PET: median SUVmax 11.3 (range: 2.3-32.5) and SUVmean 6.4 (range: 1.5-13), both significantly higher in SCCs compared to other histotypes (p=0.007 and 0.048, respectively). IHC demonstrated a median immunoreactive area covered by CD68-TAMs of 5.41% (range: 0.84-14.01%), CD8-TILs of 2.9% (range: 0.11-11.92%), PD-1 of 0.65% (range: 0.02-5.87%), and PD-L1 of 0.7% (range: 0.03-10.29%). We found a statistically significant correlation between SUVmax and SUVmean with the expression of CD68-TAMs (rho=0.31; p=0.027), CD8 TILs (rho=0.55; p>0.001), and PD-1 (rho=0.33; p=0.017 and 0.009 respectively). The other tissue markers correlated as follows: CD8 TILs and PD-1 (rho=0.45; p=0.001), CD8 TILs and PD-L1 (rho=0.41; p=0.003), CD68-TAMs and PD-L1 (rho=0.30; p=0.027), PD-1 and PD-L1 (rho=0.26; p=0.059). With respect to the outcome, only SUVmax, SUVmean and stage showed a statistically significant correlation with DFS (p=0.002, 0.004 and Conclusions The present research shows a direct association between metabolic parameters on FDG-PET and the tumor expression of immune-checkpoints and other markers of tumor-related immunity, such as PD-1 and PD-L1, CD8-TILs, and CD68-TAMs. |
| Databáze: | OpenAIRE |
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