Identification of core gene in obese type 2 diabetes patients using bioinformatics analysis
| Autor: | Hong Zhao, Zhiyong Dong, Cunchuan Wang, Xinyi Lei, Stacy A. Kujawa, NaciEmre Bolu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
CD36 Antigens
Collagen Type IV obesity bioinformatics analysis Histology Physiology CD36 Adipose tissue Type 2 diabetes Computational biology core molecular markers Diseases of the endocrine glands. Clinical endocrinology chemistry.chemical_compound Glutamate-Ammonia Ligase Adipocyte Gene expression medicine Humans QP1-981 Gene ACACB QH573-671 biology Gene Expression Profiling Computational Biology Lipid metabolism Cell Biology RC648-665 medicine.disease adipose tissue Diabetes Mellitus Type 2 chemistry biology.protein type 2 diabetes Cytology Biomarkers Software Research Article Research Paper Acetyl-CoA Carboxylase |
| Zdroj: | Adipocyte, Vol 10, Iss 1, Pp 310-321 (2021) Adipocyte article-version (VoR) Version of Record |
| ISSN: | 2162-397X 2162-3945 |
| Popis: | Objectives Adipocytes and adipocyte lipid metabolism are closely related with obesity and type 2 diabetes, but the molecular mechanism still needs further investigation. The aim of this study is to discover the adipocyte genes and pathways involved in obesity and type 2 diabetes using bioinformatics analysis. Methods The GSE27951 gene expression profile was obtained. Software and online tools (STRING, Cytoscape, BioGPS, CTD, and FunRich) were used to identify core genes.21 human subcutaneous adipose samples, with 10 from type 2 diabetic patients and 11 from normal controls, were included in these analyses. Results 184 differentially expressed genes (DEGs) including 42 up-regulated genes and 142 down-regulated genes were found to be enriched in metabolism, receptor activity, collagen type IV and glutamine biosynthesis I pathway by using the enrichment analysis. Seven hub genes were identified from the PPI network using various software (Cytoscape, STRING, BioGPS, and CTD). Four core genes (COL4A2, ACACB, GLUL, and CD36) were found to be highly expressed in subcutaneous adipose tissue of obese patients accompanying type 2 diabetes. Conclusion COL4A2, ACACB, GLUL and CD36 might be the core molecular biomarkers of obesity in patients with or without type 2 diabetes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|