Microfluidic channel with embedded SERS 2D platform for the aptamer detection of ochratoxin A
| Autor: | Eric Peyrin, Mohammadali Tabatabaei, Betty C. Galarreta, François Lagugné-Labarthet, Valérie Guieu |
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| Rok vydání: | 2012 |
| Předmět: |
Materials science
Polydimethylsiloxane Aptamer Microfluidics Nanotechnology Equipment Design Aptamers Nucleotide Microfluidic Analytical Techniques Surface Plasmon Resonance Spectrum Analysis Raman Ochratoxins Biochemistry Nanostructures Analytical Chemistry chemistry.chemical_compound symbols.namesake Nanolithography chemistry Monolayer symbols Gold Surface plasmon resonance Raman spectroscopy Plasmon |
| Zdroj: | Analytical and Bioanalytical Chemistry. 405:1613-1621 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-012-6557-7 |
| Popis: | A selective aptameric sequence is adsorbed on a two-dimensional nanostructured metallic platform optimized for surface-enhanced Raman spectroscopy (SERS) measurements. Using nanofabrication methods, a metallic nanostructure was prepared by electron-beam lithography onto a glass coverslip surface and embedded within a microfluidic channel made of polydimethylsiloxane, allowing one to monitor in situ SERS fingerprint spectra from the adsorbed molecules on the metallic nanostructures. The gold structure was designed so that its localized surface plasmon resonance matches the excitation wavelength used for the Raman measurement. This optofluidic device is then used to detect the presence of a toxin, namely ochratoxin-A (OTA), in a confined environment, using very small amounts of chemicals, and short data acquisition times, by taking advantage of the optical properties of a SERS platform to magnify the Raman signals of the aptameric monolayer system and avoiding chemical labeling of the aptamer or the OTA target. |
| Databáze: | OpenAIRE |
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