The Kupffer cell in experimental extrahepatic cholestasis in the rat—a light microscopy, immunohistochemical and electron microscopy study
| Autor: | M. E. Bailey, Penny Lovat, Derek G. D. Wight, James A. Pain, D. St. J. Collier |
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| Rok vydání: | 1986 |
| Předmět: |
Male
Pathology medicine.medical_specialty Kupffer Cells medicine.drug_class Common Bile Duct Diseases Cell Count Extrahepatic Cholestasis Biology Monoclonal antibody Major histocompatibility complex Pathology and Forensic Medicine Immunoenzyme Techniques Phagocytosis Cholestasis Histocompatibility Antigens medicine Animals Bile duct Kupffer cell Rats Inbred Strains Cholestasis Extrahepatic medicine.disease Rats Microscopy Electron Perisinusoidal space medicine.anatomical_structure biology.protein Immunohistochemistry |
| Zdroj: | The Journal of Pathology. 150:187-194 |
| ISSN: | 1096-9896 0022-3417 |
| DOI: | 10.1002/path.1711500307 |
| Popis: | Kupffer cell phagocytic function is reduced in the presence of obstructive jaundice. To investigate possible mechanisms we report a study of the rat liver in extrahepatic cholestasis, using light microscopy, immunohistochemistry and electron microscopy, Immunohistochemistry was performed with monoclonal antibodies specific for rat Kupffer cells ED 1, ED 2 and ED 3 and monoclonal antibodies directed against class II antigens of the rat major histocompatibility complex Ox 3 and Ox 6. Extrahepatic cholestasis was produced by bile duct ligation. In bile duct ligated animals light microscopy showed proliferation of bile ductules and an increase in sinusoidal cells. Immunohistochemistry with ED 1, ED 2 and ED 3 demonstrated a marked increase in the number of positive cells, but few of these cells were positive with Ox 3 and Ox 6, whereas the proliferating bile ductules were strongly positive. Electron microscopy revealed two homogeneous granular substances within the sinusoidal lumen and loss of the space of Disse. Despite a reduction in Kupffer cell phagocytic function in obstructive jaundice there is an increase in Kupffer cells, but these cells appear to be in an inactivated state as few express class II antigens on their surface. Furthermore the granular substance within the space of Disse may interfere with function. |
| Databáze: | OpenAIRE |
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