The function of serpin-2 from Eriocheir sinensis in Spiroplasma eriocheiris infection
| Autor: | Wenjing Hao, Meijun Yuan, Wei Gu, Panpan Wei, Yunting Jing, Qingguo Meng, Wen Wang, Mingxiao Ning |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Brachyura Spiroplasma Aquatic Science Serpin Real-Time Polymerase Chain Reaction Microbiology Arthropod Proteins 03 medical and health sciences Random Allocation Immune system Environmental Chemistry Animals Pathogen Serpins Messenger RNA Innate immune system biology Schneider 2 cells Gene Expression Profiling 04 agricultural and veterinary sciences General Medicine biology.organism_classification Immunity Innate Eriocheir 030104 developmental biology 040102 fisheries 0401 agriculture forestry and fisheries Hepatopancreas |
| Zdroj: | Fishshellfish immunology. 76 |
| ISSN: | 1095-9947 |
| Popis: | Serpin families classified serine protease inhibitors regulate various physiological processes. However, there is not study on the role of serpin in immune responses against Spiroplasma eriocheiris as a novel causative pathogen in the Chinese mitten crab, Eriocheir sinensis. In our study, quantitative real-time PCR (qRT-PCR) revealed that the mRNA transcripts of Esserpin-2 were ubiquitous in every tissue, relative higher expression in hepatopancreas, gill and hemocytes, while the intestine, muscle, heart and nerve showed relative lower expression. Followed by infection with S. eriocheiris, the transcripts of Esserpin-2 were significantly down-regulated from 1 d to 7 d. After double-stranded RNA injection, the transcripts of Esserpin-2 dramatically declined from 48 h to 96 h. The transcripts of proPO were found to be obviously increased after Esserpin-2 silenced, meanwhile, LGBP with no significant difference. The copy number of S. eriocheiris and subsequently the mortality of crabs in a silencing Esserpin-2 group were significantly less than control groups during infection. The subcellular localization experiment suggested that recombinant Esserpin-2 was mainly located in the cytoplasm. Finally, over-expression assay in Drosophila S2 cells indicated that Esserpin-2 could increase copies of S. eriocheiris and result in cell death. These findings demonstrated that Esserpin-2 involved in the innate immune mechanism of E. sinensis in response to S. eriocheiris infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect