A soybean calcineurin B-like protein-interacting protein kinase, GmPKS4, regulates plant responses to salt and alkali stresses
| Autor: | Xiaowei Li, Li Haiyan, Yang Li, Daqian Sun, Wang Fawei, Weican Liu, Kue Foka Idrice Carther, Quoc Viet Hoang Nguyen, Toi Ketehouli, Hu Xu, Yonggang Zhou, Si-Yu Dai |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Plant Science Alkalies Genes Plant 01 natural sciences Salt Stress 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Osmotic Pressure Stress Physiological Gene expression Protein kinase A Abscisic acid chemistry.chemical_classification Reactive oxygen species Abiotic stress Kinase Calcineurin Salt Tolerance Plants Genetically Modified Cell biology 030104 developmental biology chemistry Osmolyte Soybeans Signal transduction Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Journal of plant physiology. 256 |
| ISSN: | 1618-1328 |
| Popis: | Calcineurin B-like protein-interacting protein kinases (CIPKs) are key elements of plant abiotic stress signaling pathways. CIPKs are SOS2 (Salt Overly Sensitive 2)-like proteins (protein kinase S [PKS] proteins) which all contain a putative FISL motif. It seems that the FISL motif is found only in the SOS2 subfamily of protein kinases. In this study, the full-length cDNA of a soybean CIPK gene (GmPKS4) was isolated and was revealed to have an important role in abiotic stress responses. A qRT-PCR analysis indicated that GmPKS4 expression is upregulated under saline conditions or when exposed to alkali, salt-alkali, drought, or abscisic acid (ABA). A subcellular localization assay revealed the presence of GmPKS4 in the nucleus and cytoplasm. Further studies on the GmPKS4 promoter suggested it affects soybean resistance to various stresses. Transgenic Arabidopsis thaliana and soybean hairy roots overexpressing GmPKS4 had increased proline content as well as high antioxidant enzyme activities but decreased malondialdehyde levels following salt and salt-alkali stress treatments. Additionally, GmPKS4 overexpression activated reactive oxygen species scavenging systems, thereby minimizing damages due to oxidative and osmotic stresses. Moreover, upregulated stress-related gene expression levels were detected in lines overexpressing GmPKS4 under stress conditions. In conclusion, GmPKS4 improves soybean tolerance to salt and salt-alkali stresses. The overexpression of GmPKS4 enhances the scavenging of reactive oxygen species, osmolyte synthesis, and the transcriptional regulation of stress-related genes. |
| Databáze: | OpenAIRE |
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