From Mouse To Human : Comparative Analysis Between Grey And White Matter By Synchrotron-Fourier Transformed Infrared Microspectroscopy
| Autor: | Martin Kreuzer, Alex Perálvarez-Marín, Beatriz Almolda, Núria Benseny-Cases, Paula Sanchez-Molina, Tony Valente, Berta González, Bernardo Castellano |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
In situ
Grey matter Central nervous system lcsh:QR1-502 Infrared spectroscopy grey matter Protein aggregation Biochemistry lcsh:Microbiology White matter 03 medical and health sciences 0302 clinical medicine Lipid oxidation Species Specificity lipid oxidation Spectroscopy Fourier Transform Infrared medicine Animals Humans Gray Matter protein structure infrared spectroscopy Molecular Biology 030304 developmental biology Cerebral Cortex 0303 health sciences Principal Component Analysis Chemistry Communication Proteins Human brain central nervous system Amides Lipids White Matter Mice Inbred C57BL medicine.anatomical_structure Protein structure Protein Conformation beta-Strand 030217 neurology & neurosurgery Synchrotrons |
| Zdroj: | Biomolecules Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Biomolecules, Vol 10, Iss 1099, p 1099 (2020) |
| Popis: | Fourier Transform Infrared microspectroscopy (μFTIR) is a very useful method to analyze the biochemical properties of biological samples in situ. Many diseases affecting the central nervous system (CNS) have been studied using this method, to elucidate alterations in lipid oxidation or protein aggregation, among others. In this work, we describe in detail the characteristics between grey matter (GM) and white matter (WM) areas of the human brain by μFTIR, and we compare them with the mouse brain (strain C57BL/6), the most used animal model in neurological disorders. Our results show a clear different infrared profile between brain areas in the lipid region of both species. After applying a second derivative in the data, we established a 1.5 threshold value for the lipid/protein ratio to discriminate between GM and WM areas in non-pathological conditions. Furthermore, we demonstrated intrinsic differences of lipids and proteins by cerebral area. Lipids from GM present higher C=CH, C=O and CH3 functional groups compared to WM in humans and mice. Regarding proteins, GM present lower Amide II amounts and higher intramolecular β-sheet structure amounts with respect to WM in both species. However, the presence of intermolecular β-sheet structures, which is related to β-aggregation, was only observed in the GM of some human individuals. The present study defines the relevant biochemical properties of non-pathological human and mouse brains by μFTIR as a benchmark for future studies involving CNS pathological samples. |
| Databáze: | OpenAIRE |
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